Genotyping of the kappa-casein and beta-lactoglobulin genes in Anatolian water buffalo by PCR-RFLP

The objective of this study was to identify allele and genotype frequencies of the κ‐CN and β‐LG genes in Anatolian water buffalo. A total of 126 water buffalos from Turkey were genotyped using the PCR‐RFLP method. For gene κ‐CN, only B allele and BB genotype were observed. And for gene β‐LG, two types of alleles (A and B) and three types of genotypes were observed. The genotype frequencies of AA, AB and BB of β‐LG in Anatolian water buffalo were 0.254, 0.698 and 0.048, respectively. Surprisingly, the frequency of allele A was higher than that of allele B in contrast to world buffalo breeds.