The expression of mRNAs for the proteasome complex is developmentally regulated in the rat mesencephalon

The proteasome is a large protease complex that recognizes, unfolds and degrades ubiquitinated proteins. Evidence is now accumulating that the ubiquitin–proteasome system may play an important role in neuronal apoptosis. However, little is known about the involvement of the proteasome in neuronal death in vivo, and there has been no prior analysis of the developmental expression of proteasome subunits in brain during periods of natural and inducible apoptotic death. We therefore studied the mRNA expression levels, using Northern analysis, of a subunit from each of the three key components of the proteasome in the rat mesencephalon from E21 through development and in adulthood. We measured mRNA expression for RC6 (a subunit of 20S), p112 (a subunit of 19S) and PA28-α (a subunit of 11S). The expression of PA28-α in rat mesencephalon was highest at the earliest times studied, and then decreased at PND 21, 28 and adult, in comparison to E21 ( P