The leguminous lectin of Lonchocarpus araripensis promotes antinociception via mechanisms that include neuronal inhibition of Na+ currents
Objective and design Sodium channels are highly expressed in nociceptive sensory neurons during hypernociceptive conditions. Based on the presence of a glycosidic portion in the sodium channel β subunit associated to the antinociceptive effect of leguminous lectins via lectin domain, this study inve...
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| Veröffentlicht in: | Inflammation research 2016-09, Vol.65 (9), p.701-708 |
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| creator | Amorim, Renata Morais Ferreira Pires, Alana Freitas dos Santos-Nascimento, Tiago Cavada, Benildo S. do Nascimento, Kyria Santiago Cajazeiras, João Batista Leal-Cardoso, José Henrique Mota, Mário Rogério Lima Assreuy, Ana Maria S. |
| description | Objective and design
Sodium channels are highly expressed in nociceptive sensory neurons during hypernociceptive conditions. Based on the presence of a glycosidic portion in the sodium channel β subunit associated to the antinociceptive effect of leguminous lectins via lectin domain, this study investigated the antinociceptive activity of the lectin isolated from
Lonchocarpus araripensis
seeds (LAL) in mice behavioral models and in Na
V
current in the nociceptor of rat dorsal root ganglion (DRG).
Material/methods
LAL antinociceptive activity and the participation of opioid system, lectin domain and sodium channels were evaluated in Swiss mice models of nociception (formalin, capsaicin, hot plate, tail flick, von Frey) and in primary cultures of Wistar rats neurons of DRG (patch clamp).
Results
LAL presented inhibitory effects in the nociception induced by chemical and mechanical, but not by thermal stimuli and reduced total Na
+
current. LAL activity was inhibited by the lectin association with its binding sugar
N
-acethyl-glucosamine.
Conclusion
LAL inhibits peripheral hypernociception by mechanisms that involve the lectin domain, inflammatory mediators and Na
+
channels. The innovative inhibitory action of leguminous lectins on Na
V
current brings new insights for the investigation of sodium channels role in nociception. |
| doi_str_mv | 10.1007/s00011-016-0951-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1807275018</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1807275018</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-6bec7996fc6be56a5ea2960538f2fd7f485028e9b821ad4b4ad775f60c19551a3</originalsourceid><addsrcrecordid>eNp9kc1O3TAQha2qVflpH4AN8rISSjt24sRZItRSpCvYgMTOcpwJMUrs1E4q9RV4aoZeyrIrHx1_M5bPYexEwFcB0HzLACBEAaIuoFUk3rFDUUkoWtD370mDLItSl3DAjnJ-JFpLLT-yA9mIthRSHLKn2xH5hA_b7EPcMkm3-sDjwHcxuDE6mxaybbLJLxiyz3xJcY4rkhkIjc47XFYfA__tLZ_RjTb4PGe-jnblPrhp65EH3FIMdiJj9J3_y9Mj1_aMuy0lDGv-xD4Mdsr4-fU8Znc_vt9e_Cx2N5dXF-e7wpVVtRZ1h65p23pwpFRtFVrZ1qBKPcihb4ZKK5Aa205LYfuqq2zfNGqowYlWKWHLY_Zlv5c-8mvDvJrZZ4fTZANSBEZoaGSjQGhCxR51KeaccDBL8rNNf4wA81KB2VdgqALzUoEBmjl9Xb91M_ZvE_8yJ0DugUxX4QGTeYxbomzyf7Y-A00qlMM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1807275018</pqid></control><display><type>article</type><title>The leguminous lectin of Lonchocarpus araripensis promotes antinociception via mechanisms that include neuronal inhibition of Na+ currents</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Amorim, Renata Morais Ferreira ; Pires, Alana Freitas ; dos Santos-Nascimento, Tiago ; Cavada, Benildo S. ; do Nascimento, Kyria Santiago ; Cajazeiras, João Batista ; Leal-Cardoso, José Henrique ; Mota, Mário Rogério Lima ; Assreuy, Ana Maria S.</creator><creatorcontrib>Amorim, Renata Morais Ferreira ; Pires, Alana Freitas ; dos Santos-Nascimento, Tiago ; Cavada, Benildo S. ; do Nascimento, Kyria Santiago ; Cajazeiras, João Batista ; Leal-Cardoso, José Henrique ; Mota, Mário Rogério Lima ; Assreuy, Ana Maria S.</creatorcontrib><description>Objective and design
Sodium channels are highly expressed in nociceptive sensory neurons during hypernociceptive conditions. Based on the presence of a glycosidic portion in the sodium channel β subunit associated to the antinociceptive effect of leguminous lectins via lectin domain, this study investigated the antinociceptive activity of the lectin isolated from
Lonchocarpus araripensis
seeds (LAL) in mice behavioral models and in Na
V
current in the nociceptor of rat dorsal root ganglion (DRG).
Material/methods
LAL antinociceptive activity and the participation of opioid system, lectin domain and sodium channels were evaluated in Swiss mice models of nociception (formalin, capsaicin, hot plate, tail flick, von Frey) and in primary cultures of Wistar rats neurons of DRG (patch clamp).
Results
LAL presented inhibitory effects in the nociception induced by chemical and mechanical, but not by thermal stimuli and reduced total Na
+
current. LAL activity was inhibited by the lectin association with its binding sugar
N
-acethyl-glucosamine.
Conclusion
LAL inhibits peripheral hypernociception by mechanisms that involve the lectin domain, inflammatory mediators and Na
+
channels. The innovative inhibitory action of leguminous lectins on Na
V
current brings new insights for the investigation of sodium channels role in nociception.</description><identifier>ISSN: 1023-3830</identifier><identifier>EISSN: 1420-908X</identifier><identifier>DOI: 10.1007/s00011-016-0951-0</identifier><identifier>PMID: 27193121</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Allergology ; Analgesics - pharmacology ; Analgesics - therapeutic use ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Capsaicin ; Dermatology ; Fabaceae ; Formaldehyde ; Ganglia, Spinal - drug effects ; Ganglia, Spinal - physiology ; Hot Temperature ; Immunology ; Lectins - pharmacology ; Lectins - therapeutic use ; Male ; Mice ; Neurology ; Neurons - drug effects ; Neurons - physiology ; Nociception - drug effects ; Original Research Paper ; Pain - drug therapy ; Pharmacology/Toxicology ; Physical Stimulation ; Rats, Wistar ; Rheumatology ; Seeds ; Sodium Channels - physiology</subject><ispartof>Inflammation research, 2016-09, Vol.65 (9), p.701-708</ispartof><rights>Springer International Publishing 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-6bec7996fc6be56a5ea2960538f2fd7f485028e9b821ad4b4ad775f60c19551a3</citedby><cites>FETCH-LOGICAL-c344t-6bec7996fc6be56a5ea2960538f2fd7f485028e9b821ad4b4ad775f60c19551a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00011-016-0951-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00011-016-0951-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27193121$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amorim, Renata Morais Ferreira</creatorcontrib><creatorcontrib>Pires, Alana Freitas</creatorcontrib><creatorcontrib>dos Santos-Nascimento, Tiago</creatorcontrib><creatorcontrib>Cavada, Benildo S.</creatorcontrib><creatorcontrib>do Nascimento, Kyria Santiago</creatorcontrib><creatorcontrib>Cajazeiras, João Batista</creatorcontrib><creatorcontrib>Leal-Cardoso, José Henrique</creatorcontrib><creatorcontrib>Mota, Mário Rogério Lima</creatorcontrib><creatorcontrib>Assreuy, Ana Maria S.</creatorcontrib><title>The leguminous lectin of Lonchocarpus araripensis promotes antinociception via mechanisms that include neuronal inhibition of Na+ currents</title><title>Inflammation research</title><addtitle>Inflamm. Res</addtitle><addtitle>Inflamm Res</addtitle><description>Objective and design
Sodium channels are highly expressed in nociceptive sensory neurons during hypernociceptive conditions. Based on the presence of a glycosidic portion in the sodium channel β subunit associated to the antinociceptive effect of leguminous lectins via lectin domain, this study investigated the antinociceptive activity of the lectin isolated from
Lonchocarpus araripensis
seeds (LAL) in mice behavioral models and in Na
V
current in the nociceptor of rat dorsal root ganglion (DRG).
Material/methods
LAL antinociceptive activity and the participation of opioid system, lectin domain and sodium channels were evaluated in Swiss mice models of nociception (formalin, capsaicin, hot plate, tail flick, von Frey) and in primary cultures of Wistar rats neurons of DRG (patch clamp).
Results
LAL presented inhibitory effects in the nociception induced by chemical and mechanical, but not by thermal stimuli and reduced total Na
+
current. LAL activity was inhibited by the lectin association with its binding sugar
N
-acethyl-glucosamine.
Conclusion
LAL inhibits peripheral hypernociception by mechanisms that involve the lectin domain, inflammatory mediators and Na
+
channels. The innovative inhibitory action of leguminous lectins on Na
V
current brings new insights for the investigation of sodium channels role in nociception.</description><subject>Allergology</subject><subject>Analgesics - pharmacology</subject><subject>Analgesics - therapeutic use</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Capsaicin</subject><subject>Dermatology</subject><subject>Fabaceae</subject><subject>Formaldehyde</subject><subject>Ganglia, Spinal - drug effects</subject><subject>Ganglia, Spinal - physiology</subject><subject>Hot Temperature</subject><subject>Immunology</subject><subject>Lectins - pharmacology</subject><subject>Lectins - therapeutic use</subject><subject>Male</subject><subject>Mice</subject><subject>Neurology</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>Nociception - drug effects</subject><subject>Original Research Paper</subject><subject>Pain - drug therapy</subject><subject>Pharmacology/Toxicology</subject><subject>Physical Stimulation</subject><subject>Rats, Wistar</subject><subject>Rheumatology</subject><subject>Seeds</subject><subject>Sodium Channels - physiology</subject><issn>1023-3830</issn><issn>1420-908X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1O3TAQha2qVflpH4AN8rISSjt24sRZItRSpCvYgMTOcpwJMUrs1E4q9RV4aoZeyrIrHx1_M5bPYexEwFcB0HzLACBEAaIuoFUk3rFDUUkoWtD370mDLItSl3DAjnJ-JFpLLT-yA9mIthRSHLKn2xH5hA_b7EPcMkm3-sDjwHcxuDE6mxaybbLJLxiyz3xJcY4rkhkIjc47XFYfA__tLZ_RjTb4PGe-jnblPrhp65EH3FIMdiJj9J3_y9Mj1_aMuy0lDGv-xD4Mdsr4-fU8Znc_vt9e_Cx2N5dXF-e7wpVVtRZ1h65p23pwpFRtFVrZ1qBKPcihb4ZKK5Aa205LYfuqq2zfNGqowYlWKWHLY_Zlv5c-8mvDvJrZZ4fTZANSBEZoaGSjQGhCxR51KeaccDBL8rNNf4wA81KB2VdgqALzUoEBmjl9Xb91M_ZvE_8yJ0DugUxX4QGTeYxbomzyf7Y-A00qlMM</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Amorim, Renata Morais Ferreira</creator><creator>Pires, Alana Freitas</creator><creator>dos Santos-Nascimento, Tiago</creator><creator>Cavada, Benildo S.</creator><creator>do Nascimento, Kyria Santiago</creator><creator>Cajazeiras, João Batista</creator><creator>Leal-Cardoso, José Henrique</creator><creator>Mota, Mário Rogério Lima</creator><creator>Assreuy, Ana Maria S.</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160901</creationdate><title>The leguminous lectin of Lonchocarpus araripensis promotes antinociception via mechanisms that include neuronal inhibition of Na+ currents</title><author>Amorim, Renata Morais Ferreira ; Pires, Alana Freitas ; dos Santos-Nascimento, Tiago ; Cavada, Benildo S. ; do Nascimento, Kyria Santiago ; Cajazeiras, João Batista ; Leal-Cardoso, José Henrique ; Mota, Mário Rogério Lima ; Assreuy, Ana Maria S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-6bec7996fc6be56a5ea2960538f2fd7f485028e9b821ad4b4ad775f60c19551a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Allergology</topic><topic>Analgesics - pharmacology</topic><topic>Analgesics - therapeutic use</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Capsaicin</topic><topic>Dermatology</topic><topic>Fabaceae</topic><topic>Formaldehyde</topic><topic>Ganglia, Spinal - drug effects</topic><topic>Ganglia, Spinal - physiology</topic><topic>Hot Temperature</topic><topic>Immunology</topic><topic>Lectins - pharmacology</topic><topic>Lectins - therapeutic use</topic><topic>Male</topic><topic>Mice</topic><topic>Neurology</topic><topic>Neurons - drug effects</topic><topic>Neurons - physiology</topic><topic>Nociception - drug effects</topic><topic>Original Research Paper</topic><topic>Pain - drug therapy</topic><topic>Pharmacology/Toxicology</topic><topic>Physical Stimulation</topic><topic>Rats, Wistar</topic><topic>Rheumatology</topic><topic>Seeds</topic><topic>Sodium Channels - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amorim, Renata Morais Ferreira</creatorcontrib><creatorcontrib>Pires, Alana Freitas</creatorcontrib><creatorcontrib>dos Santos-Nascimento, Tiago</creatorcontrib><creatorcontrib>Cavada, Benildo S.</creatorcontrib><creatorcontrib>do Nascimento, Kyria Santiago</creatorcontrib><creatorcontrib>Cajazeiras, João Batista</creatorcontrib><creatorcontrib>Leal-Cardoso, José Henrique</creatorcontrib><creatorcontrib>Mota, Mário Rogério Lima</creatorcontrib><creatorcontrib>Assreuy, Ana Maria S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Inflammation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amorim, Renata Morais Ferreira</au><au>Pires, Alana Freitas</au><au>dos Santos-Nascimento, Tiago</au><au>Cavada, Benildo S.</au><au>do Nascimento, Kyria Santiago</au><au>Cajazeiras, João Batista</au><au>Leal-Cardoso, José Henrique</au><au>Mota, Mário Rogério Lima</au><au>Assreuy, Ana Maria S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The leguminous lectin of Lonchocarpus araripensis promotes antinociception via mechanisms that include neuronal inhibition of Na+ currents</atitle><jtitle>Inflammation research</jtitle><stitle>Inflamm. Res</stitle><addtitle>Inflamm Res</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>65</volume><issue>9</issue><spage>701</spage><epage>708</epage><pages>701-708</pages><issn>1023-3830</issn><eissn>1420-908X</eissn><abstract>Objective and design
Sodium channels are highly expressed in nociceptive sensory neurons during hypernociceptive conditions. Based on the presence of a glycosidic portion in the sodium channel β subunit associated to the antinociceptive effect of leguminous lectins via lectin domain, this study investigated the antinociceptive activity of the lectin isolated from
Lonchocarpus araripensis
seeds (LAL) in mice behavioral models and in Na
V
current in the nociceptor of rat dorsal root ganglion (DRG).
Material/methods
LAL antinociceptive activity and the participation of opioid system, lectin domain and sodium channels were evaluated in Swiss mice models of nociception (formalin, capsaicin, hot plate, tail flick, von Frey) and in primary cultures of Wistar rats neurons of DRG (patch clamp).
Results
LAL presented inhibitory effects in the nociception induced by chemical and mechanical, but not by thermal stimuli and reduced total Na
+
current. LAL activity was inhibited by the lectin association with its binding sugar
N
-acethyl-glucosamine.
Conclusion
LAL inhibits peripheral hypernociception by mechanisms that involve the lectin domain, inflammatory mediators and Na
+
channels. The innovative inhibitory action of leguminous lectins on Na
V
current brings new insights for the investigation of sodium channels role in nociception.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>27193121</pmid><doi>10.1007/s00011-016-0951-0</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 1023-3830 |
| ispartof | Inflammation research, 2016-09, Vol.65 (9), p.701-708 |
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| language | eng |
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| subjects | Allergology Analgesics - pharmacology Analgesics - therapeutic use Animals Biomedical and Life Sciences Biomedicine Capsaicin Dermatology Fabaceae Formaldehyde Ganglia, Spinal - drug effects Ganglia, Spinal - physiology Hot Temperature Immunology Lectins - pharmacology Lectins - therapeutic use Male Mice Neurology Neurons - drug effects Neurons - physiology Nociception - drug effects Original Research Paper Pain - drug therapy Pharmacology/Toxicology Physical Stimulation Rats, Wistar Rheumatology Seeds Sodium Channels - physiology |
| title | The leguminous lectin of Lonchocarpus araripensis promotes antinociception via mechanisms that include neuronal inhibition of Na+ currents |
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