A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue

A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue

Abstract A rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation....

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Veröffentlicht in:Placenta (Eastbourne) 2016-08, Vol.44, p.109-111
Hauptverfasser: Heywood, Wendy E, Pryce, Jeremy, Virasami, Alex, Preece, Rhian Lauren, Dezateux, Carol, Mills, Kevin, Sebire, Neil J
Format: Artikel
Sprache:eng
Schlagworte:
Chromatography, Liquid
Delivery, Obstetric
Female
Free peptide
Humans
Internal Medicine
LC-MS
Mass Spectrometry
Multivariate analysis
Obstetrics and Gynecology
Placenta
Placenta - metabolism
Placenta - pathology
Pregnancy
Proteolysis
Proteomics - methods
Specimen Handling
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Zusammenfassung:Abstract A rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation. The assay was applied to placenta samples obtained following refrigeration for varying time periods post-delivery (12 h, +24 h, +48 h and +72 h). Analysis reveals time dependant overlapping profiles for groups
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2016.04.014