Discovery and comparison of restriction patterns of Porphyra total DNAs

Total DNA is isolated from Porphyra cells which have been prepared from sea snail enzyme digests of Porphyra thalli. The crude extracts of total DNA are rapidly purified with Wizard DNA Clean-up resin by using modified washing solution. DNA bands are clearly observed in the restriction endonuclease patterns of P. yezoensis and P. haitanensis total DNAs. Both P. yezoensis and P. haitanensis have specific DNA bands in the restriction patterns of EcoRI, PastI and HaeIII. According to the band pattern of total DNA, the discrimination between P. yezoensis and P. haitanensis is very easy especially in the restriction pattern of HaeIII. The comparative results indicate that the band pattern of total DNA is a new type and reliable DNA marker for identification and classification of Porphyra species.