Molecular characterization of the gonadal kisspeptin system: Cloning, tissue distribution, gene expression analysis and localization in sablefish ( Anoplopoma fimbria )

Highlights • Four kisspeptin system mRNAs identified in sablefish ( Anoplopoma fimbria ). • Ovary and ovulated eggs were primary sites of kiss2 gene expression. • kiss1 and gpr54 mRNAs were generally detectable in gonad by qPCR but extremely low. • kiss2 mRNA localized to cytoplasm of oocytes in pri...

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Veröffentlicht in:	General and comparative endocrinology 2016-01, Vol.225, p.212-223
Hauptverfasser:	Fairgrieve, Marian R, Shibata, Yasushi, Smith, Elizabeth K, Hayman, Edward S, Luckenbach, J. Adam
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Anoplopoma fimbria Brain - metabolism Cloning, Molecular Endocrinology & Metabolism Freshwater Gonadal sex differentiation Gonads - metabolism Kisspeptin system Kisspeptins - genetics Kisspeptins - metabolism Marine Ovarian development Perciformes - metabolism Primary oocyte growth Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism Reproduction RNA, Messenger - genetics Sex Differentiation - physiology Teleost Teleostei
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Zusammenfassung:	Highlights • Four kisspeptin system mRNAs identified in sablefish ( Anoplopoma fimbria ). • Ovary and ovulated eggs were primary sites of kiss2 gene expression. • kiss1 and gpr54 mRNAs were generally detectable in gonad by qPCR but extremely low. • kiss2 mRNA localized to cytoplasm of oocytes in primary growth. • Ovarian kiss2 implicated in oogenesis and/or embryogenesis, not sex differentiation.
ISSN:	0016-6480 1095-6840
DOI:	10.1016/j.ygcen.2015.07.015