Synthesis of Didocosahexaenoylphosphatidylserine

1,2-Di-O-isopropylideneglycerophosphorochloridate prepared from isopropylindene glycerol and phosphorus oxychloride, was allowed to react with Z-l-serine-N-phthalimidomethyl ester to obtain a derivative of phosphatidylserine. Then, after the isopropylidene group was removed by Amberlite IR-120 (H + ), and the phosphate group was also blocked as a Ba-salt, this derivative was coupled with docosahexaenoic acid, applying the method of activated ester. Removal of both protective groups of serine was finally done by dry hydrogen chloride in chloroform.