SIMULTANEOUS DETECTION OF VAIRIMORPHA INVICTAE (MICROSPORIDIA: BURENELLIDAE) AND THELOHANIA SOLENOPSAE (MICROSPORIDIA: THELOHANIIDAE) IN FIRE ANTS BY PCR

A PCR-based method capable of detecting Thelohania solenopsae and/or Vairimorpha invictae infection in the red imported fire ant, Solenopsis invicta, was developed. Multiplex PCR allows simultaneous detection of both species of microsporidia in a single reaction.

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Veröffentlicht in:The Florida entomologist 2004-03, Vol.87 (1), p.85-87
Hauptverfasser: Valles, Steven M, Oi, David H, Briano, Juan A, Williams, David F
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container_title The Florida entomologist
container_volume 87
creator Valles, Steven M
Oi, David H
Briano, Juan A
Williams, David F
description A PCR-based method capable of detecting Thelohania solenopsae and/or Vairimorpha invictae infection in the red imported fire ant, Solenopsis invicta, was developed. Multiplex PCR allows simultaneous detection of both species of microsporidia in a single reaction.
doi_str_mv 10.1653/0015-4040(2004)087[0085:SDOVIM]2.0.CO;2
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source JSTOR Open Access Journals
subjects Ants
detection
DNA primers
entomopathogenic protozoa
Fire ants
Formicidae
Insect colonies
Insect genetics
Microsporidia
mixed infection
nucleotide sequences
pathogen identification
Pathogens
Polymerase chain reaction
ribosomal DNA
rRNA genes
Scientific Notes
Solenopsis invicta
Thelohania
Thelohania solenopsae
Vairimorpha
Vairimorpha invictae
title SIMULTANEOUS DETECTION OF VAIRIMORPHA INVICTAE (MICROSPORIDIA: BURENELLIDAE) AND THELOHANIA SOLENOPSAE (MICROSPORIDIA: THELOHANIIDAE) IN FIRE ANTS BY PCR
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