SIMULTANEOUS DETECTION OF VAIRIMORPHA INVICTAE (MICROSPORIDIA: BURENELLIDAE) AND THELOHANIA SOLENOPSAE (MICROSPORIDIA: THELOHANIIDAE) IN FIRE ANTS BY PCR

SIMULTANEOUS DETECTION OF VAIRIMORPHA INVICTAE (MICROSPORIDIA: BURENELLIDAE) AND THELOHANIA SOLENOPSAE (MICROSPORIDIA: THELOHANIIDAE) IN FIRE ANTS BY PCR

A PCR-based method capable of detecting Thelohania solenopsae and/or Vairimorpha invictae infection in the red imported fire ant, Solenopsis invicta, was developed. Multiplex PCR allows simultaneous detection of both species of microsporidia in a single reaction.

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Bibliographische Detailangaben
Veröffentlicht in:The Florida entomologist 2004-03, Vol.87 (1), p.85-87
Hauptverfasser: Valles, Steven M, Oi, David H, Briano, Juan A, Williams, David F
Format: Artikel
Sprache:eng
Schlagworte:
Ants
detection
DNA primers
entomopathogenic protozoa
Fire ants
Formicidae
Insect colonies
Insect genetics
Microsporidia
mixed infection
nucleotide sequences
pathogen identification
Pathogens
Polymerase chain reaction
ribosomal DNA
rRNA genes
Scientific Notes
Solenopsis invicta
Thelohania
Thelohania solenopsae
Vairimorpha
Vairimorpha invictae
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Beschreibung
Zusammenfassung:A PCR-based method capable of detecting Thelohania solenopsae and/or Vairimorpha invictae infection in the red imported fire ant, Solenopsis invicta, was developed. Multiplex PCR allows simultaneous detection of both species of microsporidia in a single reaction.
ISSN:0015-4040
1938-5102
DOI:10.1653/0015-4040(2004)087[0085:SDOVIM]2.0.CO;2