Presence of plasmids in pseudomonas isolated from ornamental fish

Following the analysis of the sanitary condition of production in fish farms from the state of Morelos in Mexico, which have used antibiotics continuously in order to prevent bacterial infection since all the fish farms have presented outbreaks, six different species of the Pseudomonadaceae family: Pseudomonas cepaciae, Pseudomonas diminuta, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas sutzerii and Pseudomonas vesicularis were isolated, purified and identified. These bacteria are pathogenic to both humans and fish. The alkaline lysis technique was used to obtain DNA from these bacteria. Antibiotic resistant plasmids of very high molecular weight were obtained from all of them. Base pair number was determined for all plasmids. We demonstrated the use of antibiotics as preventative medicine against bacterial infections in all of the farms sampled. The objective of this study was to identify the presence of plasmids that are resistant to antibiotics in bacteria from the Pseudomonadaceae family from ornamental fish from the Cichlidae, Poecilidae and Cyprinidae families, cultivated in fish farm from the state of Morelos, Mexico.Original Abstract: Despues de haber efectuado el analisis de las condiciones sanitarias de produccion de granjas piscicolas del estado de Morelos, Mexico, que han usado sistematicamente antibioticos para prevenir y controlar las infecciones de origen bacteriano sobre los cultivos, todas las granjas han presentado epizootias, se aislaron, purificaron e identificaron seis especies de bacterias pertenecientes a la familia pseudomonadaceae: Pseudomonas cepaciae, Pseudomonas diminuta, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas sutzerii y Pseudomonas vesicularis, son patogenas del ser humano y de peces. Mediante la tecnica de extraccion alcalina, se determino la presencia de plasmidos resistentes a antibioticos, de alto peso molecular, en todas las cepas. Se determino el numero de bases para cada plasmido. Se comprobo el uso de antibioticos como medida preventiva de infecciones bacterianas en las granjas en donde se efectuaron los muestreos. El objetivo del presente estudio fue comprobar la presencia de plasmidos resistentes a antibioticos en bacterias de la familia pseudomonadaceae de peces de ornato pertenecientes a las familias de los ciclidos, poecilidos y cyprinidos, cultivados en granjas piscicolas de Morelos, Mexico.