Fluvastatin, an HMG-CoA reductase inhibitor, facilitate adenosine production in the rat hearts via activation of ecto-5′-nucleotidase

The present study was examined whether fluvastatin, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, can increase the production of interstitial adenosine via activation of ecto-5′-nucleotidase in the ventricular myocardium, with use of microdialysis techniques in in situ rat hearts. Adenosine in the dialysate collected during perfusion with Tyrode's solution containing 100μM AMP (through the probe) originated from the hydrolysis of AMP catalyzed by endogenous ecto-5′-nucleotidase, so that the level of adenosine reflected the activity of ecto-5′-nucleotidase in this tissue. Fluvastatin (100μM), an inhibitor of low-density lipoprotein (LDL) oxidation, significantly increased the concentration of adenosine measured in the presence of 100μM AMP (i.e., the activity of ecto-5′-nucleotidase) by 154.7±16.0% (n=6, P