A simple high-performance liquid chromatographic method for detection of hydroxyzine in human plasma after overdose
Introduction: Hydroxyzine, a piperazine H1-receptor antagonist, is effective in generalised anxiety disorder. For toxicological purposes, a simple reversed-phase high-performance liquid chromatographic assay was developed for the detection of hydroxyzine in human plasma. Methods: A liquid–liquid pro...
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| Veröffentlicht in: | Journal of pharmacological and toxicological methods 2004-07, Vol.50 (1), p.41-44 |
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| creator | Pehourcq, F |
| description | Introduction: Hydroxyzine, a piperazine H1-receptor antagonist, is effective in generalised anxiety disorder. For toxicological purposes, a simple reversed-phase high-performance liquid chromatographic assay was developed for the detection of hydroxyzine in human plasma.
Methods: A liquid–liquid procedure was used to extract the drug from plasma in the presence of an internal standard (clothiapine). The analysis was performed on a Spherisorb S5 C
8 analytical column with UV detection.
Results: A linear response was observed over the concentration range 20–1500 ng/ml. A good accuracy (bias |
| doi_str_mv | 10.1016/j.vascn.2004.01.001 |
| format | Article |
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Methods: A liquid–liquid procedure was used to extract the drug from plasma in the presence of an internal standard (clothiapine). The analysis was performed on a Spherisorb S5 C
8 analytical column with UV detection.
Results: A linear response was observed over the concentration range 20–1500 ng/ml. A good accuracy (bias<7.3%) was achieved for all quality controls, with intraday and interday variation coefficients inferior to 8.5%. The limit of detection was 10 ng/ml, without interference of endogenous components.
Discussion: This rapid method (run time<13 min) is currently used for poison management involving hydroxyzine.</description><identifier>ISSN: 1056-8719</identifier><identifier>EISSN: 1873-488X</identifier><identifier>DOI: 10.1016/j.vascn.2004.01.001</identifier><identifier>PMID: 15233966</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Chromatography, High Pressure Liquid - methods ; Drug Overdose - blood ; Female ; High-performance liquid chromatography ; Histamine H1 Antagonists - blood ; Humans ; Hydroxyzine ; Hydroxyzine - blood ; Male ; Methods ; Overdose</subject><ispartof>Journal of pharmacological and toxicological methods, 2004-07, Vol.50 (1), p.41-44</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-9fa6f41a7eba777a88c698cc04401a88330324fd79776660b753fd95b71367193</citedby><cites>FETCH-LOGICAL-c301t-9fa6f41a7eba777a88c698cc04401a88330324fd79776660b753fd95b71367193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vascn.2004.01.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15233966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pehourcq, F</creatorcontrib><title>A simple high-performance liquid chromatographic method for detection of hydroxyzine in human plasma after overdose</title><title>Journal of pharmacological and toxicological methods</title><addtitle>J Pharmacol Toxicol Methods</addtitle><description>Introduction: Hydroxyzine, a piperazine H1-receptor antagonist, is effective in generalised anxiety disorder. For toxicological purposes, a simple reversed-phase high-performance liquid chromatographic assay was developed for the detection of hydroxyzine in human plasma.
Methods: A liquid–liquid procedure was used to extract the drug from plasma in the presence of an internal standard (clothiapine). The analysis was performed on a Spherisorb S5 C
8 analytical column with UV detection.
Results: A linear response was observed over the concentration range 20–1500 ng/ml. A good accuracy (bias<7.3%) was achieved for all quality controls, with intraday and interday variation coefficients inferior to 8.5%. The limit of detection was 10 ng/ml, without interference of endogenous components.
Discussion: This rapid method (run time<13 min) is currently used for poison management involving hydroxyzine.</description><subject>Adult</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Drug Overdose - blood</subject><subject>Female</subject><subject>High-performance liquid chromatography</subject><subject>Histamine H1 Antagonists - blood</subject><subject>Humans</subject><subject>Hydroxyzine</subject><subject>Hydroxyzine - blood</subject><subject>Male</subject><subject>Methods</subject><subject>Overdose</subject><issn>1056-8719</issn><issn>1873-488X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVJaT7aX1AoOuVmd7TySvIhhxDSJhDopYXehFYaxVpsy5HsJZtfX6W7kFtOMwPPvMM8hHxlUDNg4vu23plsx3oF0NTAagD2gZwxJXnVKPX3pPSwFpWSrD0l5zlvAYC3rPlETtl6xXkrxBnJ1zSHYeqRduGxqyZMPqbBjBZpH56W4KjtUhzMHB-Tmbpg6YBzFx0tGHU4o51DHGn0tNu7FJ_3L2FEGkbaLSWFTr3Jg6HGz5ho3GFyMeNn8tGbPuOXY70gf37c_r65qx5-_by_uX6oLAc2V603wjfMSNwYKaVRyopWWQtNA6xMnANfNd7JVkohBGzkmnvXrjeScVGe5hfk8pA7pfi0YJ71ELLFvjcjxiVrpoA1UkEB-QG0Keac0OsphcGkvWagX13rrf7vWr-61sB0cV22vh3jl82A7m3nKLcAVwcAy5O7gElnG7CodSEVb9rF8O6Bf0JTkoc</recordid><startdate>200407</startdate><enddate>200407</enddate><creator>Pehourcq, F</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>200407</creationdate><title>A simple high-performance liquid chromatographic method for detection of hydroxyzine in human plasma after overdose</title><author>Pehourcq, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-9fa6f41a7eba777a88c698cc04401a88330324fd79776660b753fd95b71367193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Drug Overdose - blood</topic><topic>Female</topic><topic>High-performance liquid chromatography</topic><topic>Histamine H1 Antagonists - blood</topic><topic>Humans</topic><topic>Hydroxyzine</topic><topic>Hydroxyzine - blood</topic><topic>Male</topic><topic>Methods</topic><topic>Overdose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pehourcq, F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of pharmacological and toxicological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pehourcq, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple high-performance liquid chromatographic method for detection of hydroxyzine in human plasma after overdose</atitle><jtitle>Journal of pharmacological and toxicological methods</jtitle><addtitle>J Pharmacol Toxicol Methods</addtitle><date>2004-07</date><risdate>2004</risdate><volume>50</volume><issue>1</issue><spage>41</spage><epage>44</epage><pages>41-44</pages><issn>1056-8719</issn><eissn>1873-488X</eissn><abstract>Introduction: Hydroxyzine, a piperazine H1-receptor antagonist, is effective in generalised anxiety disorder. For toxicological purposes, a simple reversed-phase high-performance liquid chromatographic assay was developed for the detection of hydroxyzine in human plasma.
Methods: A liquid–liquid procedure was used to extract the drug from plasma in the presence of an internal standard (clothiapine). The analysis was performed on a Spherisorb S5 C
8 analytical column with UV detection.
Results: A linear response was observed over the concentration range 20–1500 ng/ml. A good accuracy (bias<7.3%) was achieved for all quality controls, with intraday and interday variation coefficients inferior to 8.5%. The limit of detection was 10 ng/ml, without interference of endogenous components.
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Chromatography, High Pressure Liquid - methods Drug Overdose - blood Female High-performance liquid chromatography Histamine H1 Antagonists - blood Humans Hydroxyzine Hydroxyzine - blood Male Methods Overdose |
| title | A simple high-performance liquid chromatographic method for detection of hydroxyzine in human plasma after overdose |
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