A simple high-performance liquid chromatographic method for detection of hydroxyzine in human plasma after overdose

Introduction: Hydroxyzine, a piperazine H1-receptor antagonist, is effective in generalised anxiety disorder. For toxicological purposes, a simple reversed-phase high-performance liquid chromatographic assay was developed for the detection of hydroxyzine in human plasma. Methods: A liquid–liquid procedure was used to extract the drug from plasma in the presence of an internal standard (clothiapine). The analysis was performed on a Spherisorb S5 C 8 analytical column with UV detection. Results: A linear response was observed over the concentration range 20–1500 ng/ml. A good accuracy (bias