Morphometric analysis of cryofixed muscular tissue for intraoperative consultation

ABSTRACT For diagnostic purposes, cryofixation of tissues is a daily routine technique to investigate rapidly about the presence of tumours during a surgical procedure in patients. We performed morphometric analysis of cryofixed muscular tissues according to different techniques. About 1,000 muscle...

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Veröffentlicht in:	Microscopy research and technique 2016-03, Vol.79 (3), p.155-161
Hauptverfasser:	Bianciardi, Giorgio, Pontenani, Francesca, Vassallo, Loretta, Tacchini, Damiana, Buonsanti, Michele, Tripodi, Sergio
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cattle Cell Nucleus - chemistry cryofixation Cryopreservation - methods cryostat cryostat morphometry Cryostats Cytological Techniques - methods Fibers fractal analysis Fractals freezing tissue freezing tissue cryofixation human pathology Humans Image Processing, Computer-Assisted - methods Intraoperative Care intraoperative consultation Liquid nitrogen Microscopy morphometry Muscle Fibers, Skeletal - chemistry Muscle Fibers, Skeletal - cytology Muscles - cytology Nuclei Samples Shrinkage Statistical analysis Statistical methods
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Zusammenfassung:	ABSTRACT For diagnostic purposes, cryofixation of tissues is a daily routine technique to investigate rapidly about the presence of tumours during a surgical procedure in patients. We performed morphometric analysis of cryofixed muscular tissues according to different techniques. About 1,000 muscle fibers and 1,493 nuclei, were automatically examined. After freezing, ice tissue interfaces shrinkage of the cells were present. Liquid isopentane or liquid nitrogen produced a statistical increase of fractal dimension, D, of the ice‐tissue interfaces, P
ISSN:	1059-910X 1097-0029
DOI:	10.1002/jemt.22616