H sub(2)-driven biotransformation of n-octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O sub(2)-tolerant hydrogenase and a P450 monooxygenase

H sub(2)-driven biotransformation of n-octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O sub(2)-tolerant hydrogenase and a P450 monooxygenase

An in vivo biotransformation system is presented that affords the hydroxylation of n-octane to 1-octanol on the basis of NADH-dependent CYP153A monooxygenase and NAD super(+)-reducing hydrogenase heterologously synthesized in a bacterial host. The hydrogenase sustains H sub(2)-driven NADH cofactor r...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2015-10, Vol.51 (90), p.16173-16175
Hauptverfasser: Lonsdale, Thomas H, Lauterbach, Lars, Honda Malca, Sumire, Nestl, Bettina M, Hauer, Bernhard, Lenz, Oliver
Format: Artikel
Sprache:eng
Schlagworte:
Bacteria
Biotransformation
Hydrogenase
Hydroxylation
NADH
Pseudomonas
Recombinant
Regeneration
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Zusammenfassung:An in vivo biotransformation system is presented that affords the hydroxylation of n-octane to 1-octanol on the basis of NADH-dependent CYP153A monooxygenase and NAD super(+)-reducing hydrogenase heterologously synthesized in a bacterial host. The hydrogenase sustains H sub(2)-driven NADH cofactor regeneration even in the presence of O sub(2), the co-substrate of monooxygenase.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc06078h