A microfluidic indirect competitive immunoassay for multiple and sensitive detection of testosterone in serum and urine
We demonstrate a microfluidic-based indirect competitive chemiluminescence enzyme immunoassay (MIC) for multiple, sensitive, reliable and rapid detection of testosterone in human serum and urine samples. As MIC can detect biomarkers in a cost-effective and easy-to-operate manner, it may have great p...
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| Veröffentlicht in: | Analyst (London) 2016-01, Vol.141 (3), p.815-819 |
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| container_title | Analyst (London) |
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| creator | Wang, Huashan Li, Juanjuan Zhang, Xiaoqing Hu, Binfeng Liu, Yang Zhang, Lin Cha, Ruitao Sun, Jiashu Jiang, Xingyu |
| description | We demonstrate a microfluidic-based indirect competitive chemiluminescence enzyme immunoassay (MIC) for multiple, sensitive, reliable and rapid detection of testosterone in human serum and urine samples. As MIC can detect biomarkers in a cost-effective and easy-to-operate manner, it may have great potential for clinical diagnosis and point-of-care testing (POCT).
A microfluidic-based chemiluminescence immunoassay is developed for multiple and sensitive detection of testosterone in human serum and urine samples. |
| doi_str_mv | 10.1039/c5an01835h |
| format | Article |
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A microfluidic-based chemiluminescence immunoassay is developed for multiple and sensitive detection of testosterone in human serum and urine samples.</description><subject>Animals</subject><subject>Biomarkers</subject><subject>Cattle</subject><subject>Chemiluminescence</subject><subject>Goats</subject><subject>Horseradish Peroxidase - chemistry</subject><subject>Human</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Immunoenzyme Techniques - methods</subject><subject>Limit of Detection</subject><subject>Luminescent Measurements</subject><subject>Luminol - chemistry</subject><subject>Microfluidic Analytical Techniques - methods</subject><subject>Microfluidics</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Serums</subject><subject>Testosterone</subject><subject>Testosterone - blood</subject><subject>Testosterone - urine</subject><subject>Urine</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFPHCEUh4mpqVvtpXcbjqbJ2McAs8xxs7G1iamXep4wzCPSDLACU-N_L-tae-yJvPC9Ly-_HyGfGFwy4P1XI3UApri8PyIrxjvRSNmqd2QFALxpOylOyIecf9eRgYT35KTtFIiew4o8bqh3JkU7L25yhrowuYSmUBP9Dosr7g9S5_0Sos5ZP1EbE_XLXNxuRqrDRDOGfMAmLHXTxUCjpQVziblgiqEKQsXS4l8WluQCnpFjq-eMH1_fU3L37erX9rq5uf3-Y7u5aYwAVRqrhcWuhVG2a255K5joJQoce9trULI3hnXSCNWPymqj5aTNaAz0NY6Wj4qfkouDd5fiw1JvGrzLBudZB4xLHpgCEOt9jP9H11311mj36JcDWpPLOaEddsl5nZ4GBsNeNmzl5udLJ9cV_vzqXUaP0xv6t4QKnB-AlM3b779S-TMSypMu</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Wang, Huashan</creator><creator>Li, Juanjuan</creator><creator>Zhang, Xiaoqing</creator><creator>Hu, Binfeng</creator><creator>Liu, Yang</creator><creator>Zhang, Lin</creator><creator>Cha, Ruitao</creator><creator>Sun, Jiashu</creator><creator>Jiang, Xingyu</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20160101</creationdate><title>A microfluidic indirect competitive immunoassay for multiple and sensitive detection of testosterone in serum and urine</title><author>Wang, Huashan ; Li, Juanjuan ; Zhang, Xiaoqing ; Hu, Binfeng ; Liu, Yang ; Zhang, Lin ; Cha, Ruitao ; Sun, Jiashu ; Jiang, Xingyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-fa4fe620b5273f3241495e4eb9f9a0859cc165c489b8faca5dacbcc0918323b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Biomarkers</topic><topic>Cattle</topic><topic>Chemiluminescence</topic><topic>Goats</topic><topic>Horseradish Peroxidase - chemistry</topic><topic>Human</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Immunoenzyme Techniques - methods</topic><topic>Limit of Detection</topic><topic>Luminescent Measurements</topic><topic>Luminol - chemistry</topic><topic>Microfluidic Analytical Techniques - methods</topic><topic>Microfluidics</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Serums</topic><topic>Testosterone</topic><topic>Testosterone - blood</topic><topic>Testosterone - urine</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Huashan</creatorcontrib><creatorcontrib>Li, Juanjuan</creatorcontrib><creatorcontrib>Zhang, Xiaoqing</creatorcontrib><creatorcontrib>Hu, Binfeng</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Zhang, Lin</creatorcontrib><creatorcontrib>Cha, Ruitao</creatorcontrib><creatorcontrib>Sun, Jiashu</creatorcontrib><creatorcontrib>Jiang, Xingyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Huashan</au><au>Li, Juanjuan</au><au>Zhang, Xiaoqing</au><au>Hu, Binfeng</au><au>Liu, Yang</au><au>Zhang, Lin</au><au>Cha, Ruitao</au><au>Sun, Jiashu</au><au>Jiang, Xingyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A microfluidic indirect competitive immunoassay for multiple and sensitive detection of testosterone in serum and urine</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>141</volume><issue>3</issue><spage>815</spage><epage>819</epage><pages>815-819</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>We demonstrate a microfluidic-based indirect competitive chemiluminescence enzyme immunoassay (MIC) for multiple, sensitive, reliable and rapid detection of testosterone in human serum and urine samples. As MIC can detect biomarkers in a cost-effective and easy-to-operate manner, it may have great potential for clinical diagnosis and point-of-care testing (POCT).
A microfluidic-based chemiluminescence immunoassay is developed for multiple and sensitive detection of testosterone in human serum and urine samples.</abstract><cop>England</cop><pmid>26804930</pmid><doi>10.1039/c5an01835h</doi><tpages>5</tpages></addata></record> |
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| ispartof | Analyst (London), 2016-01, Vol.141 (3), p.815-819 |
| issn | 0003-2654 1364-5528 |
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| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Biomarkers Cattle Chemiluminescence Goats Horseradish Peroxidase - chemistry Human Humans Immunoassay Immunoenzyme Techniques - methods Limit of Detection Luminescent Measurements Luminol - chemistry Microfluidic Analytical Techniques - methods Microfluidics Serum Albumin, Bovine - chemistry Serums Testosterone Testosterone - blood Testosterone - urine Urine |
| title | A microfluidic indirect competitive immunoassay for multiple and sensitive detection of testosterone in serum and urine |
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