A microfluidic indirect competitive immunoassay for multiple and sensitive detection of testosterone in serum and urine

We demonstrate a microfluidic-based indirect competitive chemiluminescence enzyme immunoassay (MIC) for multiple, sensitive, reliable and rapid detection of testosterone in human serum and urine samples. As MIC can detect biomarkers in a cost-effective and easy-to-operate manner, it may have great potential for clinical diagnosis and point-of-care testing (POCT). A microfluidic-based chemiluminescence immunoassay is developed for multiple and sensitive detection of testosterone in human serum and urine samples.