Hydrazide functionalized monodispersed silica microspheres: a novel probe with tunable selectivity for a versatile enrichment of phosphopeptides with different numbers of phosphorylation sites in MS analysis

Hydrazide functionalized monodispersed silica microspheres: a novel probe with tunable selectivity for a versatile enrichment of phosphopeptides with different numbers of phosphorylation sites in MS analysis

Hydrazide functionalized monodispersed silica microspheres (HFMSM) were developed for the enrichment of phosphopeptides for the first time. With the aid of the tunable selectivity of HFMSM, global enrichment or fractionation of phosphopeptides with different numbers of phosphorylation sites could be...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2016-01, Vol.52 (6), p.1162-1165
Hauptverfasser: Xu, Linnan, Ma, Wen, Shen, Sensen, Li, Liping, Bai, Yu, Liu, Huwei
Format: Artikel
Sprache:eng
Schlagworte:
Enrichment
Fractionation
Hydrazides
Microscopy, Electron, Scanning
Microspheres
Modulation
Molecular Probes
Phosphopeptides - chemistry
Phosphorylation
Selectivity
Silicon dioxide
Silicon Dioxide - chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Zusammenfassung:Hydrazide functionalized monodispersed silica microspheres (HFMSM) were developed for the enrichment of phosphopeptides for the first time. With the aid of the tunable selectivity of HFMSM, global enrichment or fractionation of phosphopeptides with different numbers of phosphorylation sites could be realized by a simple modulation of the concentrations of formic acid in buffers.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc07941a