MISER chiral supercritical fluid chromatography for high throughput analysis of enantiopurity

•Use of MISER (Multiple Injections in a Single Experimental Run) in supercritical fluid chromatography.•Ultrafast separations combined with fast autosampler injection times allow analysis of a 96 well plate in about 33min.•User-friendly method for injector programming is shown for two recent SFC instruments. MISER chromatographic analysis (Multiple Injections in a Single Experimental Run) using supercritical fluid chromatography (SFC) with pressurized carbon dioxide-based eluents is well suited to the high throughput analysis of enantiopurity. SFC is currently the preferred method for fast enantiopurity analysis, with analysis times of only a few seconds achievable in some cases. Injector programming using both the Agilent Infinity and Shimadzu Nexera UC instruments permitted MISER SFC experiments to be performed. Several case studies are presented, showcasing the power and versatility of the technique, with ‘plate analysis times’ (the time required for analysis of enantiopurity of 96 samples) of less than 33–34min achievable in the best cases.