Enzyme link immuno assay for early detection of pregnancy associated glycoprotein's in African dwarf goat
A pregnancy test based on detection of the goat pregnancy associated glycoprotein (caPAG) by the ELISA method was designed and carried out on serum from slaughtered goats. The objective of the study was to describe the ability of this test to detect pregnancy associated glycoprotein and also their a...
Gespeichert in:
| Veröffentlicht in: | International journal of innovation and applied studies 2015-07, Vol.12 (2), p.505-505 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 505 |
|---|---|
| container_issue | 2 |
| container_start_page | 505 |
| container_title | International journal of innovation and applied studies |
| container_volume | 12 |
| creator | Nana, F C N Tume, C Daouda Dandji, M B S Zoli, A P Beckers, J F |
| description | A pregnancy test based on detection of the goat pregnancy associated glycoprotein (caPAG) by the ELISA method was designed and carried out on serum from slaughtered goats. The objective of the study was to describe the ability of this test to detect pregnancy associated glycoprotein and also their ability to discriminate between pregnant and non-pregnant females of African dwarf goats. The test was evaluated and compared to a post-mortem clinical pregnancy examination. In order to perform the test, rabbits were immunized with caPAG initially purified from cotyledons collected from pregnant goat. The antibodies obtained were biotinylated, titrated, and used in a Sandwich ELISA to detect caPAG in goats sera. Blood samples as well as informations related to each animal were collected on 506 slaughtered goats, and the sera obtained were tested for the presence of caPAG. The ELISA test results obtained from the 506 goats were different from those of the clinical examination, with 226 seropositives against 221 gestation with clinical diagnostic. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_1800448639</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1800448639</sourcerecordid><originalsourceid>FETCH-LOGICAL-p619-90d4c1d7a14cd9cdc7cc893f892eadebbc860ea06a17a3339798340320f50a983</originalsourceid><addsrcrecordid>eNpdjk9LwzAYh4soOOa-Q8CDXgpJk6XJcYz5BwZedi_vkjcls01m0iL101vRg-z0ew4PP56rYlHRSpWaV-L6H98Wq5xPlFLGBVNSLgq_C19Tj6Tz4Z34vh9DJJAzTMTFRBBSNxGLA5rBx0CiI-eEbYBgph8tGg8DWtJ2k4nnFAf04SETH8jGJW8gEPsJyZE2wnBX3DjoMq7-dlkcnnaH7Uu5f3t-3W725VkyXWpqhWG2BiaM1caa2hiluVO6QrB4PBolKQKVwGrgnOtaKy4or6hbU5h5WTz-3s45HyPmoel9Nth1EDCOuWGKUiGU5HpW7y_UUxxTmOMaJnVdc1HpNf8GE29j-A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1697734295</pqid></control><display><type>article</type><title>Enzyme link immuno assay for early detection of pregnancy associated glycoprotein's in African dwarf goat</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Nana, F C N ; Tume, C ; Daouda ; Dandji, M B S ; Zoli, A P ; Beckers, J F</creator><creatorcontrib>Nana, F C N ; Tume, C ; Daouda ; Dandji, M B S ; Zoli, A P ; Beckers, J F</creatorcontrib><description>A pregnancy test based on detection of the goat pregnancy associated glycoprotein (caPAG) by the ELISA method was designed and carried out on serum from slaughtered goats. The objective of the study was to describe the ability of this test to detect pregnancy associated glycoprotein and also their ability to discriminate between pregnant and non-pregnant females of African dwarf goats. The test was evaluated and compared to a post-mortem clinical pregnancy examination. In order to perform the test, rabbits were immunized with caPAG initially purified from cotyledons collected from pregnant goat. The antibodies obtained were biotinylated, titrated, and used in a Sandwich ELISA to detect caPAG in goats sera. Blood samples as well as informations related to each animal were collected on 506 slaughtered goats, and the sera obtained were tested for the presence of caPAG. The ELISA test results obtained from the 506 goats were different from those of the clinical examination, with 226 seropositives against 221 gestation with clinical diagnostic.</description><identifier>ISSN: 2028-9324</identifier><identifier>EISSN: 2028-9324</identifier><language>eng</language><publisher>Rabat: International Journal of Innovation and Applied Studies</publisher><subject>Antibodies ; Blood ; Diagnostic systems ; ELISA ; Gestation ; Glycoprotein ; Goats ; Pregnancy</subject><ispartof>International journal of innovation and applied studies, 2015-07, Vol.12 (2), p.505-505</ispartof><rights>Copyright International Journal of Innovation and Applied Studies Jul 2015</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Nana, F C N</creatorcontrib><creatorcontrib>Tume, C</creatorcontrib><creatorcontrib>Daouda</creatorcontrib><creatorcontrib>Dandji, M B S</creatorcontrib><creatorcontrib>Zoli, A P</creatorcontrib><creatorcontrib>Beckers, J F</creatorcontrib><title>Enzyme link immuno assay for early detection of pregnancy associated glycoprotein's in African dwarf goat</title><title>International journal of innovation and applied studies</title><description>A pregnancy test based on detection of the goat pregnancy associated glycoprotein (caPAG) by the ELISA method was designed and carried out on serum from slaughtered goats. The objective of the study was to describe the ability of this test to detect pregnancy associated glycoprotein and also their ability to discriminate between pregnant and non-pregnant females of African dwarf goats. The test was evaluated and compared to a post-mortem clinical pregnancy examination. In order to perform the test, rabbits were immunized with caPAG initially purified from cotyledons collected from pregnant goat. The antibodies obtained were biotinylated, titrated, and used in a Sandwich ELISA to detect caPAG in goats sera. Blood samples as well as informations related to each animal were collected on 506 slaughtered goats, and the sera obtained were tested for the presence of caPAG. The ELISA test results obtained from the 506 goats were different from those of the clinical examination, with 226 seropositives against 221 gestation with clinical diagnostic.</description><subject>Antibodies</subject><subject>Blood</subject><subject>Diagnostic systems</subject><subject>ELISA</subject><subject>Gestation</subject><subject>Glycoprotein</subject><subject>Goats</subject><subject>Pregnancy</subject><issn>2028-9324</issn><issn>2028-9324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdjk9LwzAYh4soOOa-Q8CDXgpJk6XJcYz5BwZedi_vkjcls01m0iL101vRg-z0ew4PP56rYlHRSpWaV-L6H98Wq5xPlFLGBVNSLgq_C19Tj6Tz4Z34vh9DJJAzTMTFRBBSNxGLA5rBx0CiI-eEbYBgph8tGg8DWtJ2k4nnFAf04SETH8jGJW8gEPsJyZE2wnBX3DjoMq7-dlkcnnaH7Uu5f3t-3W725VkyXWpqhWG2BiaM1caa2hiluVO6QrB4PBolKQKVwGrgnOtaKy4or6hbU5h5WTz-3s45HyPmoel9Nth1EDCOuWGKUiGU5HpW7y_UUxxTmOMaJnVdc1HpNf8GE29j-A</recordid><startdate>20150701</startdate><enddate>20150701</enddate><creator>Nana, F C N</creator><creator>Tume, C</creator><creator>Daouda</creator><creator>Dandji, M B S</creator><creator>Zoli, A P</creator><creator>Beckers, J F</creator><general>International Journal of Innovation and Applied Studies</general><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>M7S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope></search><sort><creationdate>20150701</creationdate><title>Enzyme link immuno assay for early detection of pregnancy associated glycoprotein's in African dwarf goat</title><author>Nana, F C N ; Tume, C ; Daouda ; Dandji, M B S ; Zoli, A P ; Beckers, J F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p619-90d4c1d7a14cd9cdc7cc893f892eadebbc860ea06a17a3339798340320f50a983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antibodies</topic><topic>Blood</topic><topic>Diagnostic systems</topic><topic>ELISA</topic><topic>Gestation</topic><topic>Glycoprotein</topic><topic>Goats</topic><topic>Pregnancy</topic><toplevel>online_resources</toplevel><creatorcontrib>Nana, F C N</creatorcontrib><creatorcontrib>Tume, C</creatorcontrib><creatorcontrib>Daouda</creatorcontrib><creatorcontrib>Dandji, M B S</creatorcontrib><creatorcontrib>Zoli, A P</creatorcontrib><creatorcontrib>Beckers, J F</creatorcontrib><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Engineering Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><jtitle>International journal of innovation and applied studies</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nana, F C N</au><au>Tume, C</au><au>Daouda</au><au>Dandji, M B S</au><au>Zoli, A P</au><au>Beckers, J F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme link immuno assay for early detection of pregnancy associated glycoprotein's in African dwarf goat</atitle><jtitle>International journal of innovation and applied studies</jtitle><date>2015-07-01</date><risdate>2015</risdate><volume>12</volume><issue>2</issue><spage>505</spage><epage>505</epage><pages>505-505</pages><issn>2028-9324</issn><eissn>2028-9324</eissn><abstract>A pregnancy test based on detection of the goat pregnancy associated glycoprotein (caPAG) by the ELISA method was designed and carried out on serum from slaughtered goats. The objective of the study was to describe the ability of this test to detect pregnancy associated glycoprotein and also their ability to discriminate between pregnant and non-pregnant females of African dwarf goats. The test was evaluated and compared to a post-mortem clinical pregnancy examination. In order to perform the test, rabbits were immunized with caPAG initially purified from cotyledons collected from pregnant goat. The antibodies obtained were biotinylated, titrated, and used in a Sandwich ELISA to detect caPAG in goats sera. Blood samples as well as informations related to each animal were collected on 506 slaughtered goats, and the sera obtained were tested for the presence of caPAG. The ELISA test results obtained from the 506 goats were different from those of the clinical examination, with 226 seropositives against 221 gestation with clinical diagnostic.</abstract><cop>Rabat</cop><pub>International Journal of Innovation and Applied Studies</pub><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2028-9324 |
| ispartof | International journal of innovation and applied studies, 2015-07, Vol.12 (2), p.505-505 |
| issn | 2028-9324 2028-9324 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1800448639 |
| source | EZB-FREE-00999 freely available EZB journals |
| subjects | Antibodies Blood Diagnostic systems ELISA Gestation Glycoprotein Goats Pregnancy |
| title | Enzyme link immuno assay for early detection of pregnancy associated glycoprotein's in African dwarf goat |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T11%3A38%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enzyme%20link%20immuno%20assay%20for%20early%20detection%20of%20pregnancy%20associated%20glycoprotein's%20in%20African%20dwarf%20goat&rft.jtitle=International%20journal%20of%20innovation%20and%20applied%20studies&rft.au=Nana,%20F%20C%20N&rft.date=2015-07-01&rft.volume=12&rft.issue=2&rft.spage=505&rft.epage=505&rft.pages=505-505&rft.issn=2028-9324&rft.eissn=2028-9324&rft_id=info:doi/&rft_dat=%3Cproquest%3E1800448639%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1697734295&rft_id=info:pmid/&rfr_iscdi=true |