Enzyme link immuno assay for early detection of pregnancy associated glycoprotein's in African dwarf goat

A pregnancy test based on detection of the goat pregnancy associated glycoprotein (caPAG) by the ELISA method was designed and carried out on serum from slaughtered goats. The objective of the study was to describe the ability of this test to detect pregnancy associated glycoprotein and also their ability to discriminate between pregnant and non-pregnant females of African dwarf goats. The test was evaluated and compared to a post-mortem clinical pregnancy examination. In order to perform the test, rabbits were immunized with caPAG initially purified from cotyledons collected from pregnant goat. The antibodies obtained were biotinylated, titrated, and used in a Sandwich ELISA to detect caPAG in goats sera. Blood samples as well as informations related to each animal were collected on 506 slaughtered goats, and the sera obtained were tested for the presence of caPAG. The ELISA test results obtained from the 506 goats were different from those of the clinical examination, with 226 seropositives against 221 gestation with clinical diagnostic.