An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures

An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures

We have developed an alternative methodology for determining the stabilities of DNA hairpin structures based on a specially designed oligonucleotide template and the T4 DNA ligase. This method was systematically tested by evaluation of DNA hairpin stabilities when varying the loop size and the natur...

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Veröffentlicht in:Bulletin of the Chemical Society of Japan 2015, Vol.88 (9), p.1314-1316
Hauptverfasser: Zhang, Hao, Ba, Sai, Co Co, Sarajane, Yiqin Lee, Jasmine, Guo, Juanjuan, Ye, Ruijuan, Liang, Zhaoxun, Huang, Dejian, Li, Tianhu
Format: Artikel
Sprache:eng
Schlagworte:
Deoxyribonucleic acid
Gene sequencing
Methodology
Oligonucleotides
Stability
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Zusammenfassung:We have developed an alternative methodology for determining the stabilities of DNA hairpin structures based on a specially designed oligonucleotide template and the T4 DNA ligase. This method was systematically tested by evaluation of DNA hairpin stabilities when varying the loop size and the nature of the loop sequence.
ISSN:0009-2673
1348-0634
DOI:10.1246/bcsj.20150120