Transcriptomes of Teak (Tectona grandis, L.f) in Vegetative to Generative Transition Stage Development

This study aimed to develop the expressed gene database to characterize the floral transcriptome in teak. The two subtracted cDNA libraries were constructed from teak bud tissues. Libraries were sequenced using Illumina MiSeq technology, which generated 3,778,316 in vegetative and 3,701,878 in generative paired-end reads sequences. The sequences combined QC tested, trimmed, and assembled de novo and conducted using CLCGenomics Workbench. The sequence reads assembled de novo into 87,365 transcript contigs consisting of 42,435,728 bases with N50 of 498bp. The 64,961 assembled contigs exhibited similarity BLASTN to Arabidopsis thaliana database. The assembled contigs were annotated through high stringency BLASTX analysis to proteome of A. thaliana. Distribution of contigs abundance between vegetative and generative stages was analyzed using the DEGseq approach. The numbers of contigs distribution are 24,730 in vegetative, 28,912 in generative, and 33,723 in transition stage. The functionally protein datasets were characterized by gene ontology annotation and KEGG metabolic pathways assignments for the result of DEG analysis.