Generation of a human iPSC line from a patient with Leigh syndrome

Generation of a human iPSC line from a patient with Leigh syndrome

Human iPSC line LND554SV.3 was generated from heteroplasmic fibroblasts of a patient with Leigh syndrome carrying a mutation in the MT-ND5 gene (m.13513G>A; p.D393N). Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sen...

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Veröffentlicht in:Stem cell research 2016-01, Vol.16 (1), p.63-66
Hauptverfasser: Galera, Teresa, Zurita, Francisco, González-Páramos, Cristina, Moreno-Izquierdo, Ana, Fraga, Mario F., Fernández, Agustin F., Garesse, Rafael, Gallardo, M. Esther
Format: Artikel
Sprache:eng
Schlagworte:
Cell Culture Techniques - methods
Cell Differentiation
Cell Line
Humans
Induced pluripotent stem cells
Induced Pluripotent Stem Cells - cytology
iPS cells
iPSC
Karyotyping
Leigh Disease - pathology
Leigh syndrome
MT-ND5
Sendai virus
Sequence Analysis, DNA
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Zusammenfassung:Human iPSC line LND554SV.3 was generated from heteroplasmic fibroblasts of a patient with Leigh syndrome carrying a mutation in the MT-ND5 gene (m.13513G>A; p.D393N). Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2015.12.005