A Stepwise Method for the Isolation of Endothelial Cells And Smooth Muscle Cells From Individual Canine Coronary Arteries

A Stepwise Method for the Isolation of Endothelial Cells And Smooth Muscle Cells From Individual Canine Coronary Arteries

Methods for the stepwise isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries are described. Both cell types can be isolated in pure culture with high yields. Dogs are a common species used in the study of atherosclerosis and coronary artery disease. Capaci...

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Veröffentlicht in:In vitro cellular & developmental biology. Animal 2003-11, Vol.39 (10), p.402-406
Hauptverfasser: Dame, M K, Yu, X, Garrido, R, Bobrowski, W, Mcduffie, JE, Murphy, H S, Albassam, M, Varani, J
Format: Artikel
Sprache:eng
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Zusammenfassung:Methods for the stepwise isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries are described. Both cell types can be isolated in pure culture with high yields. Dogs are a common species used in the study of atherosclerosis and coronary artery disease. Capacity to isolate endothelial cells and smooth muscle cells from individual canine coronary arteries should prove useful in the study of coronary artery disease.
ISSN:1071-2690
1543-706X
DOI:10.1290/1543-706X(2003)039(0402:ASMFTI)2.0.CO;2