Biodiesel production from different algal oil using immobilized pure lipase and tailor made rPichia pastoris with Cal A and Cal B genes
[Display omitted] •Method to obtain maximum oil yield from macroalgae by solvent extraction was determined.•Stable intercellular lipase producing Pichia pastoris was constructed.•Biological catalysts was used to produce biodiesel from macroalgal oil.•rPichia pastoris lipase and purified lipase perfo...
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Veröffentlicht in: | Bioresource technology 2016-08, Vol.213, p.69-78 |
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Sprache: | eng |
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•Method to obtain maximum oil yield from macroalgae by solvent extraction was determined.•Stable intercellular lipase producing Pichia pastoris was constructed.•Biological catalysts was used to produce biodiesel from macroalgal oil.•rPichia pastoris lipase and purified lipase performance was determined.
In this investigation, oil extraction was performed in marine macroalgae Gracilaria edulis, Enteromorpha compressa and Ulva lactuca. The algal biomass was characterized by Scanning Electron Microscopy and Fourier Transform-Infra Red Spectroscopy. Six different pre-treatment methods were carried out to evaluate the best method for maximum oil extraction. Optimization of extraction parameters were performed and high oil yield was obtained at temperature 55°C, time 150min, particle size 0.10mm, solvent-to-solid ratio 6:1 and agitation rate 500rpm. After optimization, 9.5%, 12.18% and 10.50 (g/g) of oil extraction yield was achieved from the respective algal biomass. The rate constant for extraction was obtained as first order kinetics, by differential method. Stable intracellular Cal A and Cal B lipase producing recombinant Pichia pastoris was constructed and used as biocatalyst for biodiesel production. Comparative analysis of lipase activity and biodiesel yield was made with immobilized Candida antarctica lipase. |
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ISSN: | 0960-8524 1873-2976 |
DOI: | 10.1016/j.biortech.2016.02.041 |