Molecular characterisation of rough strains of Vibrio cholerae isolated from diarrhoeal cases in India and their comparison to smooth strains

Sixteen of the 18 Vibrio cholerae rough strains isolated from hospitalised diarrhoea patients were found to contain O1 serotype-specific ( wbe) genes and all currently known virulence genes. Expression of the regulatory element ToxR was evident in these strains. Cholera toxin production ability of t...

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Veröffentlicht in:	FEMS microbiology letters 2004-03, Vol.232 (1), p.23-30
Hauptverfasser:	De, Keya, Ramamurthy, Thandavarayan, Faruque, Shah M, Yamasaki, Shinji, Takeda, Yoshifumi, Nair, G.Balakrish, Nandy, Ranjan K
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial diseases Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Bacteriology Bacteriophages - genetics Bacteriophages - physiology Biological and medical sciences Cholera Cholera - microbiology Cholera toxin Cholera Toxin - biosynthesis Cholera Toxin - genetics Diarrhea - microbiology DNA-Binding Proteins - biosynthesis DNA-Binding Proteins - genetics Feces - microbiology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genes, Bacterial Genomic Islands - genetics Human bacterial diseases Humans India Infectious diseases Lysogeny Medical sciences Microbiology Miscellaneous Molecular Epidemiology O Antigens - analysis O Antigens - genetics Ribotyping Rough strain Transcription Factors - biosynthesis Transcription Factors - genetics Transcription, Genetic Transduction, Genetic Tropical bacterial diseases Vibrio cholerae Vibrio cholerae - classification Vibrio cholerae - genetics Vibrio cholerae - isolation & purification Virulence Factors - analysis Virulence Factors - genetics
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Zusammenfassung:	Sixteen of the 18 Vibrio cholerae rough strains isolated from hospitalised diarrhoea patients were found to contain O1 serotype-specific ( wbe) genes and all currently known virulence genes. Expression of the regulatory element ToxR was evident in these strains. Cholera toxin production ability of the rough strains was found to be higher (c. three- to five-fold) as compared to the smooth counterparts and this was transcriptionally regulated. Strains exhibiting the rough phenotype were more amenable to the uptake of CTXφ, which led us to consider that the rough phenotype could play a role in the generation of genetic diversity among V. cholerae strains.
ISSN:	0378-1097 1574-6968
DOI:	10.1016/S0378-1097(04)00013-8