Ozone exposure induces iNOS expression and tyrosine nitration in rat aorta
The aim was to study whether ozone affects vascular endothelium by causing inducible nitric oxide synthase (iNOS) expression and tyrosine nitration. We also studied biomarkers of endothelial function. Male Wistar rats were exposed to ozone (0.25 ppm, 4 h/day) or filtered air (control, ozone
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| Veröffentlicht in: | Environmental toxicology and pharmacology 2004-05, Vol.17 (1), p.1-7 |
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| creator | Sánchez-González, Dolores J Moro, Marı́a A Castillo-Henkel, Carlos Herrera-González, Norma Hernández-Pando, Rogelio Larios-Medina, Francisco J Cobilt, Rafael Blanco, José A Pedraza-Chaverrı́, José Villanueva, Cleva |
| description | The aim was to study whether ozone affects vascular endothelium by causing inducible nitric oxide synthase (iNOS) expression and tyrosine nitration. We also studied biomarkers of endothelial function. Male Wistar rats were exposed to ozone (0.25
ppm, 4
h/day) or filtered air (control, ozone |
| doi_str_mv | 10.1016/j.etap.2004.01.002 |
| format | Article |
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ppm, 4
h/day) or filtered air (control, ozone <0.05
ppm). After ozone exposure, blood samples were taken to measure 6-keto prostaglandin F1α (6-keto PGF1α), dehydro-thromboxane B
2 (DH-TxB
2), endothelin-1 and NO
2
−/NO
3
− (NO
x
−). iNOS and nitrotyrosine were detected in aorta by immunohistochemistry. Nitrotyrosine was also detected by immunoelectromicroscopy. Control aortae failed to show either iNOS or nitrotyrosine. Time-dependent positive iNOS and nitrotyrosine cells were observed in exposed animals. Except for NO
x
−, endothelial markers decreased after 14 days of ozone exposure (
P<0.05). After 28 days of ozone, 6-keto PGF1α remained low (
P<0.05) while DH-TxB
2 increased (
P<0.05). It is concluded that ozone causes endothelial dysfunction manifested early with peroxynitrite formation and lately with changes in endothelial markers.</description><identifier>ISSN: 1382-6689</identifier><identifier>EISSN: 1872-7077</identifier><identifier>DOI: 10.1016/j.etap.2004.01.002</identifier><identifier>PMID: 21782706</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Endothelial dysfunction ; Oxidative stress ; Ozone ; Peroxynitrite</subject><ispartof>Environmental toxicology and pharmacology, 2004-05, Vol.17 (1), p.1-7</ispartof><rights>2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-6021ee7f0f4110334c1ddd9850f1b88294b78ac31c11949ea50f7847031f14e43</citedby><cites>FETCH-LOGICAL-c385t-6021ee7f0f4110334c1ddd9850f1b88294b78ac31c11949ea50f7847031f14e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1382668904000055$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21782706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sánchez-González, Dolores J</creatorcontrib><creatorcontrib>Moro, Marı́a A</creatorcontrib><creatorcontrib>Castillo-Henkel, Carlos</creatorcontrib><creatorcontrib>Herrera-González, Norma</creatorcontrib><creatorcontrib>Hernández-Pando, Rogelio</creatorcontrib><creatorcontrib>Larios-Medina, Francisco J</creatorcontrib><creatorcontrib>Cobilt, Rafael</creatorcontrib><creatorcontrib>Blanco, José A</creatorcontrib><creatorcontrib>Pedraza-Chaverrı́, José</creatorcontrib><creatorcontrib>Villanueva, Cleva</creatorcontrib><title>Ozone exposure induces iNOS expression and tyrosine nitration in rat aorta</title><title>Environmental toxicology and pharmacology</title><addtitle>Environ Toxicol Pharmacol</addtitle><description>The aim was to study whether ozone affects vascular endothelium by causing inducible nitric oxide synthase (iNOS) expression and tyrosine nitration. We also studied biomarkers of endothelial function. Male Wistar rats were exposed to ozone (0.25
ppm, 4
h/day) or filtered air (control, ozone <0.05
ppm). After ozone exposure, blood samples were taken to measure 6-keto prostaglandin F1α (6-keto PGF1α), dehydro-thromboxane B
2 (DH-TxB
2), endothelin-1 and NO
2
−/NO
3
− (NO
x
−). iNOS and nitrotyrosine were detected in aorta by immunohistochemistry. Nitrotyrosine was also detected by immunoelectromicroscopy. Control aortae failed to show either iNOS or nitrotyrosine. Time-dependent positive iNOS and nitrotyrosine cells were observed in exposed animals. Except for NO
x
−, endothelial markers decreased after 14 days of ozone exposure (
P<0.05). After 28 days of ozone, 6-keto PGF1α remained low (
P<0.05) while DH-TxB
2 increased (
P<0.05). It is concluded that ozone causes endothelial dysfunction manifested early with peroxynitrite formation and lately with changes in endothelial markers.</description><subject>Endothelial dysfunction</subject><subject>Oxidative stress</subject><subject>Ozone</subject><subject>Peroxynitrite</subject><issn>1382-6689</issn><issn>1872-7077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMorl9_wIP0JF5aZ5K2ScGLLH4i7kE9h2w6hSy77Zq04vrrTVn16GmGmWdekoexU4QMAcvLRUa9WWccIM8AMwC-ww5QSZ5KkHI39kLxtCxVNWGHISwAsBBC7bMJR6m4hPKAPc6-upYS-lx3YfCUuLYeLIXEPc9exqmnEFzXJqatk37ju-Ai3brem34cuzaJXWI635tjtteYZaCTn3rE3m5vXqf36dPs7mF6_ZRaoYo-LYEjkWygyRFBiNxiXdeVKqDBuVK8yudSGSvQIlZ5RSYupMolCGwwp1wcsfNt7tp37wOFXq9csLRcmpa6IWiUVcGLSkTw4n8QFOciPgIiyreojV8Mnhq99m5l_CZCepStF3qUrUfZGlBH2fHo7Cd_mK-o_jv5tRuBqy1AUceHI6-DddRaqp0n2-u6c__lfwO8-o8C</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Sánchez-González, Dolores J</creator><creator>Moro, Marı́a A</creator><creator>Castillo-Henkel, Carlos</creator><creator>Herrera-González, Norma</creator><creator>Hernández-Pando, Rogelio</creator><creator>Larios-Medina, Francisco J</creator><creator>Cobilt, Rafael</creator><creator>Blanco, José A</creator><creator>Pedraza-Chaverrı́, José</creator><creator>Villanueva, Cleva</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20040501</creationdate><title>Ozone exposure induces iNOS expression and tyrosine nitration in rat aorta</title><author>Sánchez-González, Dolores J ; Moro, Marı́a A ; Castillo-Henkel, Carlos ; Herrera-González, Norma ; Hernández-Pando, Rogelio ; Larios-Medina, Francisco J ; Cobilt, Rafael ; Blanco, José A ; Pedraza-Chaverrı́, José ; Villanueva, Cleva</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-6021ee7f0f4110334c1ddd9850f1b88294b78ac31c11949ea50f7847031f14e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Endothelial dysfunction</topic><topic>Oxidative stress</topic><topic>Ozone</topic><topic>Peroxynitrite</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sánchez-González, Dolores J</creatorcontrib><creatorcontrib>Moro, Marı́a A</creatorcontrib><creatorcontrib>Castillo-Henkel, Carlos</creatorcontrib><creatorcontrib>Herrera-González, Norma</creatorcontrib><creatorcontrib>Hernández-Pando, Rogelio</creatorcontrib><creatorcontrib>Larios-Medina, Francisco J</creatorcontrib><creatorcontrib>Cobilt, Rafael</creatorcontrib><creatorcontrib>Blanco, José A</creatorcontrib><creatorcontrib>Pedraza-Chaverrı́, José</creatorcontrib><creatorcontrib>Villanueva, Cleva</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Environmental toxicology and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sánchez-González, Dolores J</au><au>Moro, Marı́a A</au><au>Castillo-Henkel, Carlos</au><au>Herrera-González, Norma</au><au>Hernández-Pando, Rogelio</au><au>Larios-Medina, Francisco J</au><au>Cobilt, Rafael</au><au>Blanco, José A</au><au>Pedraza-Chaverrı́, José</au><au>Villanueva, Cleva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ozone exposure induces iNOS expression and tyrosine nitration in rat aorta</atitle><jtitle>Environmental toxicology and pharmacology</jtitle><addtitle>Environ Toxicol Pharmacol</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>17</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>1382-6689</issn><eissn>1872-7077</eissn><abstract>The aim was to study whether ozone affects vascular endothelium by causing inducible nitric oxide synthase (iNOS) expression and tyrosine nitration. We also studied biomarkers of endothelial function. Male Wistar rats were exposed to ozone (0.25
ppm, 4
h/day) or filtered air (control, ozone <0.05
ppm). After ozone exposure, blood samples were taken to measure 6-keto prostaglandin F1α (6-keto PGF1α), dehydro-thromboxane B
2 (DH-TxB
2), endothelin-1 and NO
2
−/NO
3
− (NO
x
−). iNOS and nitrotyrosine were detected in aorta by immunohistochemistry. Nitrotyrosine was also detected by immunoelectromicroscopy. Control aortae failed to show either iNOS or nitrotyrosine. Time-dependent positive iNOS and nitrotyrosine cells were observed in exposed animals. Except for NO
x
−, endothelial markers decreased after 14 days of ozone exposure (
P<0.05). After 28 days of ozone, 6-keto PGF1α remained low (
P<0.05) while DH-TxB
2 increased (
P<0.05). It is concluded that ozone causes endothelial dysfunction manifested early with peroxynitrite formation and lately with changes in endothelial markers.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21782706</pmid><doi>10.1016/j.etap.2004.01.002</doi><tpages>7</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Endothelial dysfunction Oxidative stress Ozone Peroxynitrite |
| title | Ozone exposure induces iNOS expression and tyrosine nitration in rat aorta |
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