Severe sensitivity loss in an influenza A molecular assay due to antigenic drift variants during the 2014/15 influenza season

Abstract The 2014–2015 influenza season in Belgium was dominated by the circulation of 2 influenza A(H3N2) subgroups: 3C.2a and 3C.3b. Analysis of 166 nasopharyngeal aspirates, collected in patients with respiratory illness at the start of the epidemic season, showed a decreased sensitivity for the...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2016-05, Vol.85 (1), p.42-46
Hauptverfasser:	Overmeire, Yarah, Vanlaere, Elke, Hombrouck, Anneleen, De Beenhouwer, Hans, Simons, Guus, Brink, Antoinette, Van den Abeele, Anne-Marie, Verfaillie, Charlotte, Van Acker, Jos
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigenic drift Antigenic Variation - immunology Humans Infectious Disease Influenza Influenza A virus - classification Influenza A virus - genetics Influenza A virus - immunology Influenza, Human - diagnosis Influenza, Human - epidemiology Influenza, Human - immunology Influenza, Human - virology Internal Medicine Laboratory Laboratory surveillance Molecular Diagnostic Techniques - methods Molecular Diagnostic Techniques - standards Molecular methods Mutation Phylogeny Polymerase Chain Reaction Reagent Kits, Diagnostic Reproducibility of Results RNA, Viral Seasons Sensitivity and Specificity
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Zusammenfassung:	Abstract The 2014–2015 influenza season in Belgium was dominated by the circulation of 2 influenza A(H3N2) subgroups: 3C.2a and 3C.3b. Analysis of 166 nasopharyngeal aspirates, collected in patients with respiratory illness at the start of the epidemic season, showed a decreased sensitivity for the detection of influenza A(H3N2)/3C.2a using a commercially available multiplex assay. Gene sequencing of the matrix protein showed a point mutation (C163T) leading to a mismatch with the assay probes.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2016.02.004