New cyt b gene universal primer set for forensic analysis

Highlights • The cyt b universal primers here developed have proved a high ability for species identification. • Its power is due to the binding to conserved regions flanking variable sequence. • Because the amplicons are 148 bp it is useful to amplify degraded forensic samples. • The primers design...

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Veröffentlicht in:	Forensic science international : genetics 2016-07, Vol.23, p.159-165
Hauptverfasser:	Lopez-Oceja, A, Gamarra, D, Borragan, S, Jiménez-Moreno, S, de Pancorbo, M.M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cytochrome b Cytochromes b - genetics Decapoda (Crustacea) - genetics DNA Degradation, Necrotic DNA Primers DNA, Mitochondrial - genetics Forensic Humans Mitochondrial DNA Pathology Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Species Specificity Vertebrates - genetics
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Zusammenfassung:	Highlights • The cyt b universal primers here developed have proved a high ability for species identification. • Its power is due to the binding to conserved regions flanking variable sequence. • Because the amplicons are 148 bp it is useful to amplify degraded forensic samples. • The primers designed have proved to work in species of even five different Classes.
ISSN:	1872-4973 1878-0326
DOI:	10.1016/j.fsigen.2016.05.001