Overview of fusion tags for recombinant proteins

Virtually all recombinant proteins are now prepared using fusion domains also known as “tags”. The use of tags helps to solve some serious problems: to simplify procedures of protein isolation, to increase expression and solubility of the desired protein, to simplify protein refolding and increase i...

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Veröffentlicht in:Biochemistry (Moscow) 2016-03, Vol.81 (3), p.187-200
Hauptverfasser: Kosobokova, E. N., Skrypnik, K. A., Kosorukov, V. S.
Format: Artikel
Sprache:eng
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Zusammenfassung:Virtually all recombinant proteins are now prepared using fusion domains also known as “tags”. The use of tags helps to solve some serious problems: to simplify procedures of protein isolation, to increase expression and solubility of the desired protein, to simplify protein refolding and increase its efficiency, and to prevent proteolysis. In this review, advantages and disadvantages of such fusion tags are analyzed and data on both well-known and new tags are generalized. The authors own data are also presented.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297916030019