Measurement of Individual Nanobioparticles on Microfluidic Chips by Laser Dark-field Imaging

A microfluidic-chip-based analysis platform specially tuned for characterizing individual nanobio-particles has been developed. Using this platform, both artificial nanobioparticles (polyion complex vesicles) and cell-secreted exosomes with diameter down to 50 nm can be observed. The zeta potential distribution of the exosomes was obtained by measuring the zeta potential of individual exosomes. Since the number of surface molecules on nanobioparticles can be estimated by applying a particle immunoelectrophoresis method, this platform is promising for obtaining profiles of heterogeneous nanobioparticles including nanoDDS carriers and exosomes.