A direct competitive assay-based aptasensor for sensitive determination of tetracycline residue in Honey

Tetracycline (TC) is a common antibacterial agent used for prevention and control of animal diseases. The increasing concern about TC residue in food demands high-performing analytical techniques for food quality assessment. Biosensors represent a promising tool for food safety analysis as they can fulfill some demand that the conventional methods do not attain. In this study, a novel colorimetric aptasensor was developed for sensitive detection of TC in honey. The aptasensor was based on a modified direct competitive enzyme-linked aptamer assay (dc-ELAA) scheme utilizing a 76mer single-stranded DNA (ssDNA) aptamer selected by Systematic Evolution of Ligands by Exponential Enrichment (SELEX). The optimized aptasensor showed a good limit of detection (LOD of 0.0978ng/mL), a wide linear range (0.1–1000ng/mL) toward TC in honey, with good recoveries (92.09–109.7%) in TC-spiked honey, and was compared with an indirect competitive assay-based aptasensor and validated with a standard ELISA. The biosensor based on dc-ELAA with good limit of detection and simplicity can be applied for high-throughput detection of TC in food. [Display omitted] •An aptasensor was developed to detect tetracycline in honey with high-throughput.•Detailed optimization was performed for assay conditions.•An improved operation procedure of dc-ELAA was carried out for the first time.•The aptasensor presented a good limit of detection and a wide linear range.•The ic-ELAA and dc-ELAA for TC detection in honey was compared preliminarily.