Optimization of Preparation Technology on Medicated Serum of Yinqiaotiangan in Mice

Response surface methodology (RSM) and single factor test were used to optimize preparation technology on medicated serum of Yinqiaotiangan (YQTG) in mice. The influent factors of preparing medicated serum include blood sampling time, administration times, and administration dosage. The content of chlorogenic acid in medicated serum was detected by high performance liquid chromatography (HPLC). Results showed that the optimum preparation technology of medicated serum is 33 minutes of blood sampling time, 4 times of administration times and 0.024g/mL of administration concentration. Under this condition, the concentration of chlorogenic acid reached peak 33.34 μg/mL in mice medicated serum. It is seen that the RSM method effectively and accurately optimize preparation technology on medicated serum of YQTG preparation.