A target responsive aptamer machine for label-free and sensitive non-enzymatic recycling amplification detection of ATP

Based on a new target-triggered aptamer molecular machine, a label-free and non-enzymatic target recycling amplification strategy for sensitive fluorescence detection of ATP in human serums is described. The presence of the target ATP together with the DNA fuel strand initiates the operation of the aptamer machine and leads to cyclic reuse of ATP and the release of many G-quadruplex sequences, which associate with a fluorescent dye to generate significantly amplified fluorescence signals to achieve sensitive detection of ATP. Label-free and non-enzymatic target recycling amplification detection of ATP is achieved through an aptamer machine powered by the DNA fuel.