Erythrocyte aggregation under high pressure studied by laser photometry and mathematical analysis
[Display omitted] •We investigated the effects of hydrostatic pressure on the erythrocyte aggregation.•The measurements were carried by laser photometry.•A theory relating the erythrocyte aggregation process was developed.•The theory was able to describe correctly the experimental data.•We discussed...
Gespeichert in:
Veröffentlicht in: | Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2016-04, Vol.140, p.189-195 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | [Display omitted]
•We investigated the effects of hydrostatic pressure on the erythrocyte aggregation.•The measurements were carried by laser photometry.•A theory relating the erythrocyte aggregation process was developed.•The theory was able to describe correctly the experimental data.•We discussed the physical meaning of the parameters in the theoretical equation.
The effects of hydrostatic pressure on erythrocyte aggregation have been studied by laser photometry and analysis based on a phenomenological theory. Samples were prepared by suspending swine erythrocytes in their own plasma. A high-pressure vessel consisting of a stainless-steel block with a hole to hold a sample cell and two sapphire windows to allows the passage of a He–Ne laser beam was used in the experimental setup. The suspension was stirred at 1500rpm to disperse the erythrocytes homogeneously. Immediately after reducing the stirring rate from 1500rpm to 300rpm, the transmitted light intensity (I) was recorded every 10ms under a high pressure of 40–200MPa. The value of I increased with time (t) owing to erythrocyte aggregation. From the phenomenological theory, the equation ΔI(t)=ΔIeq[1−e−Kt/(1−B(1−e−Kt))] was derived for the change in the transmitted light intensity (ΔI) due to erythrocyte aggregation, where ΔIeq is the transmitted light intensity in the steady state, K is a time constant and B is a constant that represents the ratio of the number of interaction sites on erythrocyte aggregates at time t to that in the steady state. The observed time courses of ΔI obtained at all pressures could be closely fitted to the theoretical equation. ΔIeq roughly increased with increasing pressure. On the other hand, K and B abruptly decreased above 120MPa. The growth rate of aggregates decreased above 120MPa. These results suggest a change in the mechanism of erythrocyte aggregation at approximately 120MPa. We discuss the physical meaning of the parameters. |
---|---|
ISSN: | 0927-7765 1873-4367 |
DOI: | 10.1016/j.colsurfb.2015.12.038 |