Kinetics of tRNA(Pyl) -mediated amber suppression in Escherichia coli translation reveals unexpected limiting steps and competing reactions

The utility of ribosomal incorporation of unnatural amino acids (AAs) in vivo is generally restricted by low efficiencies, even with the most widely used suppressor tRNA(Pyl) . Because of the difficulties of studying incorporation in vivo, almost nothing is known about the limiting steps after tRNA...

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Veröffentlicht in:	Biotechnology and bioengineering 2016-07, Vol.113 (7), p.1552-1559
Hauptverfasser:	Wang, Jinfan, Kwiatkowski, Marek, Forster, Anthony C
Format:	Artikel
Sprache:	eng
Schlagworte:	Escherichia coli - genetics Escherichia coli - metabolism Genes, Bacterial - genetics Genes, Suppressor Kinetics Peptide Elongation Factor Tu - genetics Peptide Elongation Factor Tu - metabolism Protein Biosynthesis - genetics RNA, Transfer, Met - genetics RNA, Transfer, Met - metabolism
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