Legionella drozanskii sp. nov., Legionella rowbothamii sp. nov. and Legionella fallonii sp. nov.: three unusual new Legionella species
AA Adeleke, BS Fields, RF Benson, MI Daneshvar, JM Pruckler, RM Ratcliff, TG Harrison, RS Weyant, RJ Birtles, D Raoult and MA Halablab Division of Life Sciences, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 8WA, UK Seven strains of Legionella-like amoebal pa...
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Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2001-05, Vol.51 (3), p.1151-1160 |
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Zusammenfassung: | AA Adeleke, BS Fields, RF Benson, MI Daneshvar, JM Pruckler, RM Ratcliff, TG Harrison, RS Weyant, RJ Birtles, D Raoult and MA Halablab
Division of Life Sciences, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 8WA, UK
Seven strains of Legionella-like amoebal pathogens (LLAPs) were
characterized on the basis of their cultural and staining characteristics,
biochemical reactions, serology, cellular fatty acids (CFAs), isoprenoid
quinone composition, total DNA relatedness, analysis of 16S rRNA and
macrophage infectivity potentiator (mip) gene sequence analyses. All seven
strains exhibited limited growth on buffered charcoal yeast extract alpha
(BCYE) agar, required cysteine for growth and contained branched-chain CFAs
and quinones typical of Legionella species. The bacilli were Gram-negative
and catalase-positive. There were varying degrees of serological
cross-reactions between these LLAP strains and other previously described
Legionella species. Results from the various tests revealed that four LLAP
strains represent three unusual new species of Legionella: Legionella
drozanskii sp. nov., type strain LLAP-1(T); Legionella rowbothamii sp.
nov., type strain LLAP-6(T); and Legionella fallonii sp. nov., type strain
LLAP-10(T). Three other LLAP strains, designated LLAP-7FL, LLAP-7NF and
LLAP-9, were shown to be members of the species Legionella lytica. The
deductions made from the phenetic characteristics of these bacteria were
consistent with the phylogenetic relationships inferred from 16S rRNA and
mip gene sequence analyses. This study is the first to speciate LLAP
strains on the basis of data including quantitative DNA hybridization. |
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ISSN: | 1466-5026 1466-5034 |
DOI: | 10.1099/00207713-51-3-1151 |