A quick colorimetric method for total lipid quantification in microalgae

Discovering microalgae with high lipid productivity are among the key milestones for achieving sustainable biodiesel production. Current methods of lipid quantification are time intensive and costly. A rapid colorimetric method based on sulfo-phospho-vanillin (SPV) reaction was developed for the quantification of microbial lipids to facilitate screening for lipid producing microalgae. This method was successfully tested on marine thraustochytrid strains and vegetable oils. The colorimetric method results correlated well with gravimetric method estimates. The new method was less time consuming than gravimetric analysis and is quantitative for lipid determination, even in the presence of carbohydrates, proteins and glycerol. •A rapid colorimetric method for lipid quantification in microalgae was developed.•Accuracy of the method was compared with gravimetric and GC methods.•Lipid contents were determined in microalgal and vegetable oils.•Cellular components such as carbohydrates, proteins did not interfere