Dictyostelium discoideum as a surrogate host–microbe model for antivirulence screening in Pseudomonas aeruginosa PAO1

Highlights • Using a virtual screening approach, compounds that inhibit Pseudomonas aeruginosa PAO1 polyphosphate kinase 1 ( Pa PPK1) in vitro were identified. • A simple high-throughput developmental assay using Dictyostelium discoideum for discovering novel antivirulence agents against P. aerugino...

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Veröffentlicht in:	International journal of antimicrobial agents 2016-05, Vol.47 (5), p.403-409
Hauptverfasser:	Bravo-Toncio, Catalina, Álvarez, Javiera A, Campos, Francisca, Ortíz-Severín, Javiera, Varas, Macarena, Cabrera, Ricardo, Lagos, Carlos F, Chávez, Francisco P
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Infective Agents - isolation & purification Anti-Infective Agents - pharmacology Antivirulence Dictyostelium - microbiology Drug Evaluation, Preclinical - methods High-Throughput Screening Assays Host–pathogen interaction In silico drug discovery Infectious Disease Models, Theoretical Polyphosphate Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - pathogenicity Pseudomonas aeruginosa - physiology Social amoeba Virulence - drug effects
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Beschreibung
Zusammenfassung:	Highlights • Using a virtual screening approach, compounds that inhibit Pseudomonas aeruginosa PAO1 polyphosphate kinase 1 ( Pa PPK1) in vitro were identified. • A simple high-throughput developmental assay using Dictyostelium discoideum for discovering novel antivirulence agents against P. aeruginosa PAO1 without side effects to the host or its beneficial bacteria was designed. • PPK1 inhibitors showed in vivo antivirulence activity toward P. aeruginosa PAO1.
ISSN:	0924-8579 1872-7913
DOI:	10.1016/j.ijantimicag.2016.02.005