The 120 592 bp IncF plasmid pRSB107 isolated from a sewage-treatment plant encodes nine different antibiotic-resistance determinants, two iron-acquisition systems and other putative virulence-associated functions

Fakultät für Biologie, Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, D-33501 Bielefeld, Germany Correspondence A. Schlüter Andreas.Schlueter{at}Genetik.Uni-Bielefeld.DE The antibiotic-multiresistance IncF plasmid pRSB107 was isolated by a transformation-based approach from activated-sludge bacteria of a wastewater-treatment plant. It confers resistance to ampicillin, penicillin G, chloramphenicol, erythromycin, kanamycin, neomycin, streptomycin, sulfonamides, tetracycline and trimethoprim and against mercuric ions. Complete sequencing of this plasmid revealed that it is 120 592 bp in size and has a G+C content of 53·1 mol%. The plasmid backbone is composed of three replicons, RepFIA, RepFIB and RepFII, which are almost identical to corresponding regions located on the F-plasmid and on R100. The three replicons encode replication initiation ( rep ) and replication control, multimer resolution ( mrs ), post-segregational killing of plasmid-free cells ( psk ) and active plasmid partitioning ( sopABC locus). Part of the F-leading region and remnants of the F-homologous DNA-transfer ( tra ) module complete the pRSB107 backbone. Plasmid pRSB107 contains a complex, highly mosaic 35 991 bp antibiotic-resistance region consisting of a Tn 21 - and a Tn 10 -derivative and a chloramphenicol-resistance module. The Tn 21 derivative is composed of a mercury-resistance region ( mer ), a Tn 4352B -like kanamycin/neomycin-resistance transposon, a streptomycin/sulfonamide-resistance module, remnants of the -lactam-resistance transposon Tn 1 , a macrolide-resistance module flanked by copies of IS 26 and IS 6100 , remnants of Tn 402 integrating a class 1 integron and the Tn 21 -specific transposition module. A truncated version of the tetracycline-resistance transposon Tn 10 and the chloramphenicol acetyltransferase gene catA complete the pRSB107 resistance region. In addition to antibiotic resistance, pRSB107 encodes the following putative virulence-associated functions: (i) an aerobactin iron-acquisition siderophore system ( iuc / iut ); (ii) a putative high-affinity Fe 2+ uptake system which was previously identified on a pathogenicity island of Yersinia pestis and in the genome of the phytopathogen Erwinia carotovora subsp. atroseptica SCRI1043; (iii) an sn -glycerol-3-phosphate transport system ( ugp ); and (iv) the virulence-associated genes vagCD having a possible function in stable plasmid inheritance. All the accessory modules are framed by insertion