Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation

The present work compares six biochemical methods for extraction of lipids from human serum. Although some organic solvents were good lipid extractors, they precipitated most of the total proteins and albumin. On the other hand, methodologies using Triton X-114 and silica were efficient for extracti...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of biochemical and biophysical methods 2004-03, Vol.58 (3), p.187-193
Hauptverfasser:	Ferraz, T.P.L, Fiúza, M.C, dos Santos, M.L.A, Pontes de Carvalho, L, Soares, N.M
Format:	Artikel
Sprache:	eng
Schlagworte:	Clinical Laboratory Techniques Detergent Humans Lipid extraction Lipids - chemistry Lipids - isolation & purification Organic solvents Polyethylene Glycols - chemistry Preservation, Biological Proteins Proteins - chemistry Sensitivity and Specificity Serum - chemistry Silica Silicon Dioxide - chemistry Solvents - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The present work compares six biochemical methods for extraction of lipids from human serum. Although some organic solvents were good lipid extractors, they precipitated most of the total proteins and albumin. On the other hand, methodologies using Triton X-114 and silica were efficient for extraction of lipids, while sparing the protein fraction.
ISSN:	0165-022X 1872-857X
DOI:	10.1016/j.jbbm.2003.10.008