Defective sirtuin-1 increases IL-4 expression through acetylation of GATA-3 in patients with severe asthma

Decreased SIRT1 activity was also positively correlated with decreased FEV1 (Fig 1, B). Because the expression of SIRT1 mRNA transcripts quantified by using quantitative RT-PCR (qRT-PCR) showed no change between groups, the reduction of SIRT1 is not due to reduced gene transcription (Fig 1, C). According to additional analysis with CD4+CD45RA+ T cells from cord blood, which were induced to differentiate into TH1 and TH2 subsets, TH1-dominant cells preincubated with sirtinol demonstrated no significant change in the ratios of IFN-γ to IL-4 and T-box transcription factor (T-bet) to GATA-3 when normalized against glyceraldehyde-3-phoshpahte dehydrogenase (GAPDH; data not shown).