Chemo-enzymatic synthesis of 3-(2-naphthyl)-l-alanine by an aminotransferase from the extreme thermophile, Thermococcus profundus

Chemo-enzymatic synthesis of 3-(2-naphthyl)-l-alanine by an aminotransferase from the extreme thermophile, Thermococcus profundus

Hyper-thermostable aminotransferase from Thermococcus profundus (MsAT) was used to synthesize 3-(2-naphthyl)-l-alanine (Nal) by transamination between its corresponding α-keto acid, 3-(2-naphthyl)pyruvate (NPA) and l-glutamate (Glu) at 70 °C. Equilibrium of this reaction was shifted toward Nal produ...

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Veröffentlicht in:Biotechnology letters 2001-04, Vol.23 (8), p.589-591
Hauptverfasser: HANZAWA, Satoshi, OE, Seigo, TOKUHISA, Kenji, KAWANO, Kazuhisa, KOBAYASHI, Tetsuo, KUDO, Toshiaki, KAKIDANI, Hitoshi
Format: Artikel
Sprache:eng
Schlagworte:
3-(2-Naphthyl)-L-alanine
3-(2-naphthyl)pyruvate
3-(2-Naphthyl)pyruvic acid
a-Keto acids
aminotransferase
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Chemical synthesis
Enzymes
Fundamental and applied biological sciences. Psychology
glutamic acid
Methods. Procedures. Technologies
Thermococcus profundus
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Zusammenfassung:Hyper-thermostable aminotransferase from Thermococcus profundus (MsAT) was used to synthesize 3-(2-naphthyl)-l-alanine (Nal) by transamination between its corresponding α-keto acid, 3-(2-naphthyl)pyruvate (NPA) and l-glutamate (Glu) at 70 °C. Equilibrium of this reaction was shifted toward Nal production due to its low solubility, giving rise to Nal precipitate. Optically pure Nal (>99% ee) was synthesized with 93% (mol mol^sup -1^) yield from 180 mM NPA and 360 mM Glu.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1010308506263