Novel microsatellite markers for Bactris gasipaes (Palmae)

Ten new polymorphic microsatellites were isolated and characterized in Bactris gasipaes using a microsatellite enrichment protocol and selective hybridization with oligonucleotide probes. The loci are highly polymorphic, with a mean of 14.6 alleles per locus and a mean expected heterozygosity of 0.8...

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Veröffentlicht in:	Molecular ecology notes 2004-12, Vol.4 (4), p.575-576
Hauptverfasser:	Rodrigues, D.P, Vinson, C, Ciampi, A.Y, Farias, I.P, Lemes, M.R, Astolfi-Filho, S, Clements, C.R
Format:	Artikel
Sprache:	eng
Schlagworte:	alleles Bactris gasipaes DNA primers genetic markers heterozygosity landrace loci microsatellite repeats microsatellites molecular sequence data nucleotide sequences peach palm polymerase chain reaction
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Beschreibung
Zusammenfassung:	Ten new polymorphic microsatellites were isolated and characterized in Bactris gasipaes using a microsatellite enrichment protocol and selective hybridization with oligonucleotide probes. The loci are highly polymorphic, with a mean of 14.6 alleles per locus and a mean expected heterozygosity of 0.83 among 62 individuals of the Pampa Hermosa landrace. These microsatellites will be useful for population genetic analysis and germplasm characterization for heart‐of‐palm breeding.
ISSN:	1471-8278 1471-8286
DOI:	10.1111/j.1471-8286.2004.00739.x