Oxygen transfer on beta-D-galactosidase production by Kluyveromyces marxianus using sugar cane molasses as carbon source

The optimal initial volumetric oxygen transfer coefficient (KLa) was 60 h(-1) for the production of beta-D-galactosidase from Kluyveromyces marxianus CDB 002, using sugar cane molasses as carbon source. At this KLa applying an agitation/aeration relationship of 700 rpm/0.66 vvm resulted in 812 U l(-1) h(-1) for beta-D-galactosidase production. This was about 50% better than a relationship of 500 rpm/2 vvm at the same KLa.