Vitamin B12 functionalized layer by layer calcium phosphate nanoparticles: A mucoadhesive and pH responsive carrier for improved oral delivery of insulin

[Display omitted] The present study investigates the potential of layer by layer coated calcium phosphate nanoparticles – for oral delivery of insulin where Vitamin B12 grafted chitosan and sodium alginate have been used as cationic and anionic polyelectrolyte respectively. The major emphasis has be...

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Veröffentlicht in:	Acta biomaterialia 2016-02, Vol.31, p.288-300
Hauptverfasser:	Verma, Ashwni, Sharma, Shweta, Gupta, Pramod Kumar, Singh, Awadhesh, Teja, B. Venkatesh, Dwivedi, Pankaj, Gupta, Girish Kumar, Trivedi, Ritu, Mishra, Prabhat Ranjan
Format:	Artikel
Sprache:	eng
Schlagworte:	Administration, Oral AFM Alginates - chemistry Animals Bioavailability Caco-2 Caco-2 Cells Calcium Phosphates - chemistry Carbodiimides - chemistry Cationic Chitosan Chitosan - chemistry Diabetes Diabetes Mellitus, Experimental - drug therapy Drug Carriers - chemistry Electrolytes - chemistry Endocytosis Flow Cytometry Fluorescein-5-isothiocyanate - chemistry Glucuronic Acid - chemistry Hexuronic Acids - chemistry Humans Hydrogen-Ion Concentration Hypoglycemia Insulin Insulin - administration & dosage Insulin - chemistry Intrinsic Factor Male Microscopy, Confocal Microscopy, Fluorescence Nanoparticles Nanoparticles - chemistry Paracellular transport Particle Size Rats Rats, Wistar Receptor mediated endocytosis Simulation TEER Uptakes Vitamin B 12 - chemistry Vitamin B12
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Zusammenfassung:	[Display omitted] The present study investigates the potential of layer by layer coated calcium phosphate nanoparticles – for oral delivery of insulin where Vitamin B12 grafted chitosan and sodium alginate have been used as cationic and anionic polyelectrolyte respectively. The major emphasis has been given on the role Vitamin B12 conjugated chitosan as cationic polyelectrolyte (VitB12-Chi) in the delivery system. VitB12-Chi conjugate was prepared by carbodiimide reaction. The formulated VirB12-Chi-CPNPs were tested for in vitro and in vivo efficacy studies carried out in Caco-2 monolayers and diabetic rats. VitB12-Chi-CPNPs with particle size
ISSN:	1742-7061 1878-7568
DOI:	10.1016/j.actbio.2015.12.017