Cyclam-functionalized carbon dots sensor for sensitive and selective detection of copper(II) ion and sulfide anion in aqueous media and its imaging in live cells
Novel Cyclam-functionalized carbon dots for highly sensitive (detection limit: 100nM for Cu2+ and 130nM for S2−) and selective detection of Cu2+ and S2− in water were prepared by surface modification of prepared carbon dots. The prepared dual-ion sensor can be reversibly switched for several times b...
Gespeichert in:
Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2016-03, Vol.224, p.298-306 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Novel Cyclam-functionalized carbon dots for highly sensitive (detection limit: 100nM for Cu2+ and 130nM for S2−) and selective detection of Cu2+ and S2− in water were prepared by surface modification of prepared carbon dots. The prepared dual-ion sensor can be reversibly switched for several times by selective addition of adequate Cu2+ or S2−, and exhibited excellent longterm photostability for Cu2+ detection (≥35 days) in aqueous media. More importantly, the CCDs sensor also displayed low cytotoxicity and can be utilized to monitor Cu2+ and S2− level change in live cells.
•The CCDs sensor was prepared by surface modification of prepared carbon dots.•The sensor can selectively sense Cu2+ and S2− in water.•The detection limit of sensor is 100nM for Cu2+ and 130nM for S2−.•The sensor has excellent longterm photostability and low cytotoxicity.•The sensor can realize monitoring of Cu2+ and S2− in live cells.
The detection of copper ion (Cu2+) and sulfide anion (S2−) is of vital importance since the abnormal level of Cu2+ or S2− can lead to many diseases. Herein, a highly sensitive and selective fluorescent sensor, 1,4,8,11-tetraazacyclotetradecane (Cyclam)-functionalized carbon dots (CCDs), has been designed, synthesized and evaluated for Cu2+ and S2−. For this nanoprobe, a specific fluorescence resonance energy transfer (FRET) process can be effectively take place between carbon dots and the surface Cu2+-Cyclam complex, the as-prepared CCDs display high sensitivity (detection limit: 100nM) and selectivity toward Cu2+ among many other metal cations (such as Mg2+, Co2+, Pb2+, Ni2+, Mn2+, Hg2+, Fe2+, Ca2+ and Zn2+) in 100% aqueous solution. Moreover, it is worth to point out that the subsequent addition of S2− can extract Cu2+ from the CCDs-Cu2+ complex and recover the fluorescence of carbon dots, the detection limit for S2− can reach to 130nM in the aqueous medium. And no statistically significant interference was observed among the other 10 anions (HCO3−, SO42−, NO3−, Cl−, CO32−, S2O32−, F−, Br−, HPO4−, ClO4−) for S2− through the study. In addition, the novel type of multifunctional fluorescent sensor has a relatively wide pH range (pH 4–10). At the same time, it exhibited remarkable longterm fluorescence stability (≥35 days) for Cu2+ detection. In addition, this nanoprobe exhibits very low cytotoxicity and can easily permeate the cell membrane and realize Cu2+ and S2− monitoring and imaging in live cells. Therefore, this novel approach can be used in various |
---|---|
ISSN: | 0925-4005 1873-3077 |
DOI: | 10.1016/j.snb.2015.10.046 |