An impedimetric biosensor to test neat serum for dengue diagnosis

NS1, a non-structural dengue protein, has been used as a biomarker for the detection of viremia in dengue diagnosis. In this work, an impedimetric label-free immunosensor based on an anti-NS1 modified gold electrode was developed. To immobilize anti-NS1, a mixed self-assembled monolayer consisting of 11-mercaptoundecanoic acid (for covalent anti-NS1 attachment) and 6-mercaptohexanol (as a spacer) was prepared. The impedance spectra were recorded in the presence of a redox probe ([Fe(CN)6]3−/4−) to monitor changes in charge-transfer resistance associated with target binding both in PBS and neat serum. A calibration curve was constructed in order to obtain analytical parameters. NS1 was diluted in PBS to final concentrations in the linear range of 0.01–2.00μgmL−1 with a sensitivity of 14.1percentagedecade−1 (R∼0.99) and a limit of detection (LOD) of 3ngmL−1. A linear range 0.01–1.00μgmL−1 of NS1 diluted in neat serum was also obtained (R∼0.98) with a sensitivity of 10.4percentagedecade−1 and a LOD of 30ngmL−1. The results open new insights into the potential use of this biosensor for point-of-care and bedside applications for dengue diagnosis.