A Fast and Simple Method for the Measurement of Total Antioxidant Potential and a Fingerprint of Antioxidants

The goal of this paper is to demonstrate a new approach to the chromatographic total antioxidant potential (TAP) assay and chromatographic fingerprints of honeys. The analyte is analyzed using reversed-phase high-performance liquid chromatography with electrochemical (amperometric) detection. The TAP measure was the total surface area of all recorded chromatographic peaks (on the amperometric detector in the oxidation potential range) on the chromatogram. The proposed assay is superior to already described assays because TAP can easily be related to the different potentials of working electrodes, as well as different groups of compounds separated on the column. The fingerprinting of antioxidants (antiradicals) has been applied for the quality assessment of honeys.